Purification of camel liver catalase by zinc chelate affinity chromatography and pH gradient elution: An enzyme with interesting properties

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dc.authorid57190409749
dc.authorid6507478596
dc.authorid56400331100
dc.authorid7004132795
dc.contributor.authorChafik A.
dc.contributor.authorEssamadi A.
dc.contributor.authorÇelik S.Y.
dc.contributor.authorMavi A.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:43:13Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:43:13Z
dc.date.issued2017
dc.departmentBayburt Üniversitesien_US
dc.description.abstractClimate change and increasing temperatures are global concerns. Camel (Camelus dromedarius) lives most of its life under high environmental stress in the desert and represent ideal model for studying desert adaptation among mammals. Catalase plays a key role in protecting cells against oxidative stress. For the first time, catalase from camel liver was purified to homogeneity by zinc chelate affinity chromatography using pH gradient elution, a better separation was obtained. A purification fold of 201.81 with 1.17% yield and a high specific activity of 1132539.37 U/mg were obtained. The native enzyme had a molecular weight of 268 kDa and was composed of four subunits of equal size (65 kDa). The enzyme showed optimal activity at a temperature of 45 °C and pH 7.2. Thiol reagents, ?-Mercaptoethanol and D,L-Dithiothreitol, inhibited the enzyme activity. The enzyme was inhibited by Al3+, Cd2+ and Mg2+, whereas Ca2+, Co2+ and Ni2+ stimulated the catalase activity. Reduced glutathione has no effect on catalase activity. The Km and Vmax of the enzyme for hydrogen peroxide were 37.31 mM and 6185157 U/mg, respectively. Sodium azide inhibited the enzyme noncompetitively with Ki value of 14.43 ?M, the IC50 was found to be 16.71 ?M. The properties of camel catalase were different comparing to those of mammalian species. Relatively higher molecular weight, higher optimum temperature, protection of reduced glutathione from hydrogen peroxide oxidation and higher affinity for hydrogen peroxide and sodium azide, these could be explained by the fact that camel is able to live in the intense environmental stress in the desert. © 2017 Elsevier B.V.en_US
dc.identifier.doi10.1016/j.jchromb.2017.10.052
dc.identifier.endpage111
dc.identifier.issn1570-0232
dc.identifier.pmid29100758en_US
dc.identifier.scopus2-s2.0-85032912902en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage104
dc.identifier.urihttps://dx.doi.org/10.1016/j.jchromb.2017.10.052
dc.identifier.urihttps://hdl.handle.net/20.500.12403/453
dc.identifier.volume1070
dc.identifier.wosWOS:000417661900015en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAffinity chromatography
dc.subjectCamel
dc.subjectCamelus dromedarius
dc.subjectCatalase
dc.subjectDesert
dc.subjectpH gradient elution
dc.subjectAffinity chromatography
dc.subjectChelation
dc.subjectChromatography
dc.subjectClimate change
dc.subjectEnzymes
dc.subjectHydrogen peroxide
dc.subjectLandforms
dc.subjectMammals
dc.subjectMolecular weight
dc.subjectOxidation
dc.subjectPeptides
dc.subjectPeroxides
dc.subjectpH
dc.subjectPurification
dc.subjectSodium
dc.subjectZinc
dc.subjectCamel
dc.subjectCamelus dromedarius
dc.subjectCatalase
dc.subjectDesert
dc.subjectpH gradient elution
dc.subjectEnzyme activity
dc.subjectcalcium ion
dc.subjectcatalase
dc.subjectcobalt
dc.subjectdithiothreitol
dc.subjectglutathione
dc.subjecthydrogen peroxide
dc.subjectmercaptoethanol
dc.subjectnickel
dc.subjectsodium azide
dc.subjectzinc
dc.subjectcatalase
dc.subjectdodecyl sulfate sodium
dc.subjectedetic acid
dc.subjectenzyme inhibitor
dc.subjectaffinity chromatography
dc.subjectArticle
dc.subjectcamel
dc.subjectcontrolled study
dc.subjectelution
dc.subjectenzyme activity
dc.subjectenzyme inhibition
dc.subjectenzyme purification
dc.subjectgradient elution
dc.subjectIC50
dc.subjectliver
dc.subjectmolecular weight
dc.subjectnonhuman
dc.subjectoxidation
dc.subjectpH
dc.subjectpriority journal
dc.subjecttemperature
dc.subjectaffinity chromatography
dc.subjectanimal
dc.subjectantagonists and inhibitors
dc.subjectchemistry
dc.subjectenzymology
dc.subjectisolation and purification
dc.subjectliver
dc.subjectmetabolism
dc.subjectpH
dc.subjectprocedures
dc.subjectAnimals
dc.subjectCamelus
dc.subjectCatalase
dc.subjectChromatography, Affinity
dc.subjectEdetic Acid
dc.subjectEnzyme Inhibitors
dc.subjectHydrogen-Ion Concentration
dc.subjectLiver
dc.subjectSodium Dodecyl Sulfate
dc.subjectTemperature
dc.subjectAffinity chromatography
dc.subjectCamel
dc.subjectCamelus dromedarius
dc.subjectCatalase
dc.subjectDesert
dc.subjectpH gradient elution
dc.subjectAffinity chromatography
dc.subjectChelation
dc.subjectChromatography
dc.subjectClimate change
dc.subjectEnzymes
dc.subjectHydrogen peroxide
dc.subjectLandforms
dc.subjectMammals
dc.subjectMolecular weight
dc.subjectOxidation
dc.subjectPeptides
dc.subjectPeroxides
dc.subjectpH
dc.subjectPurification
dc.subjectSodium
dc.subjectZinc
dc.subjectCamel
dc.subjectCamelus dromedarius
dc.subjectCatalase
dc.subjectDesert
dc.subjectpH gradient elution
dc.subjectEnzyme activity
dc.subjectcalcium ion
dc.subjectcatalase
dc.subjectcobalt
dc.subjectdithiothreitol
dc.subjectglutathione
dc.subjecthydrogen peroxide
dc.subjectmercaptoethanol
dc.subjectnickel
dc.subjectsodium azide
dc.subjectzinc
dc.subjectcatalase
dc.subjectdodecyl sulfate sodium
dc.subjectedetic acid
dc.subjectenzyme inhibitor
dc.subjectaffinity chromatography
dc.subjectArticle
dc.subjectcamel
dc.subjectcontrolled study
dc.subjectelution
dc.subjectenzyme activity
dc.subjectenzyme inhibition
dc.subjectenzyme purification
dc.subjectgradient elution
dc.subjectIC50
dc.subjectliver
dc.subjectmolecular weight
dc.subjectnonhuman
dc.subjectoxidation
dc.subjectpH
dc.subjectpriority journal
dc.subjecttemperature
dc.subjectaffinity chromatography
dc.subjectanimal
dc.subjectantagonists and inhibitors
dc.subjectchemistry
dc.subjectenzymology
dc.subjectisolation and purification
dc.subjectliver
dc.subjectmetabolism
dc.subjectpH
dc.subjectprocedures
dc.subjectAnimals
dc.subjectCamelus
dc.subjectCatalase
dc.subjectChromatography, Affinity
dc.subjectEdetic Acid
dc.subjectEnzyme Inhibitors
dc.subjectHydrogen-Ion Concentration
dc.subjectLiver
dc.subjectSodium Dodecyl Sulfate
dc.subjectTemperature
dc.titlePurification of camel liver catalase by zinc chelate affinity chromatography and pH gradient elution: An enzyme with interesting propertiesen_US
dc.typeArticleen_US

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