Biological control of green mould on mandarin fruit through the combined use of antagonistic yeasts
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press Inc Elsevier Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Penicillium digitatum is the primary cause of green mould in mandarins during the postharvest period, mainly controlled through synthetic fungicides. This study evaluated the biocontrol of green mould on mandarin fruit with three antagonistic yeasts (Hanseniaspora uvarum, Meyerozyma guilliermondii, and Metschnikowia aff. pul-cherrima P01A016), alone or in combination, by elucidating their possible mechanisms of action. All yeast cultures alone (73.85 % -80.64 %) and their combinations (78.40 % -83.18 %) reduced the mycelial growth of green mold in vitro. M. aff. pulcherrima reduced the disease incidence and lesion diameters by 75.5 % and 91.3 %, respectively, demonstrating the highest biocontrolling activity alone. Meyerozyma guilliermondii showed the highest biofilm formation (OD 0.93 +/- 0.01) and antifungal activity (71.13 %) through volatile organic com-pounds (VOCs), while H. uvarum displayed cell-wall degrading beta-1,3 glucanase activity together with mycoci-nogenic and VOCs activity. All tested yeasts showed chitinase, protease, and leucine arylamidase activity. Among dual combinations, M. guilliermondii and M. aff. pulcherrima (M-1) were the most compatible, while M. aff. pulcherrima and H. uvarum (M-3) had the least compatibility. The combined application of these three yeasts resulted in synergistic co-operation, demonstrating the highest biocontrolling efficacy in vitro and in vivo. Inte-grating multiple mechanisms of action in yeasts could create a hurdle approach for inhibiting green mould in citrus as a green alternative to synthetic fungicides. Thus, our study demonstrated that combining biocontrol yeasts can result in beneficial consortia for the eco-friendly and sustainable management of P.digitatum-caused postharvest diseases of mandarins in both the wounded and intact mandarin fruits.
Açıklama
Anahtar Kelimeler
Penicillium digitatum, Citrus, Biological control, Postharvest, Mycocinogenic activity, Biofungicide
Kaynak
Biological Control
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
180
