Changes in carbonic anhydrase activity and gene expression of Hsp70 in rainbow trout (Oncorhynchus mykiss) muscle after exposure to some metals

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dc.authorid23502381000
dc.authorid6603903192
dc.authorid29067602900
dc.authorid7006097499
dc.authorid55537752500
dc.contributor.authorSöyüt H.
dc.contributor.authorBeydemir S.
dc.contributor.authorCeyhun S.B.
dc.contributor.authorErdo?an O.
dc.contributor.authorKaya E.D.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:44:34Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:44:34Z
dc.date.issued2012
dc.departmentBayburt Üniversitesien_US
dc.description.abstractThe effects of some heavy metals Co(II), Cu(II), Zn(II), and Ag(I) on carbonic anhydrase (CA) activity from rainbow trout (RT) muscle were investigated. Moreover, the effects of these metals on the expression of heat shock protein 70 (Hsp70) gene in muscle tissue were examined by real-time quantitative PCR (RT-PCR) in muscle tissue aft er exposure to the metals at the end of 6, 12, 24, and 48 h. CA was purified with a specific activity of 2300 EU mg-1, a yield of 19%, and 1080-fold. The molecular weights (Mw) of subunit and native enzyme were approximately 30 and 31 kDa, respectively. Optimum pH, stable pH, optimum temperature, activation energy (E?), activation enthalpy (?H), and Q10 (the difference in activity of enzyme caused by the increment of 10 oC) value were determined. Apparent Michaelis constant (Km), maximum reaction rate (Vmax), and turnover rate of the enzyme (Kcat) values were 1.29 mM, 0.17 ?mol min-1, and 28.8 s-1, respectively. The catalytic efficiency (Kcat/Km) was 22.3. The heavy metals decreased in vitro CA activity. Inhibition mechanisms of the metal ions were noncompetitive, except for the Co(II) ion, which was competitive. The expression of the Hsp70 gene was increased in the presence of the metal ions. The expression level at the end of 48 h was the highest for all of the metals. Consequently, the in vitro inhibition rank order was determined as Co(II) > Zn(II) > Cu(II) > Ag(I). Interestingly, Ag(I) was the most effective metal ion on Hsp70 gene expression. © TÜBİTAK.en_US
dc.identifier.doi10.3906/vet-1010-3
dc.identifier.endpage508
dc.identifier.issn1300-0128
dc.identifier.issue5
dc.identifier.scopus2-s2.0-84871533434en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage499
dc.identifier.urihttps://dx.doi.org/10.3906/vet-1010-3
dc.identifier.urihttps://hdl.handle.net/20.500.12403/884
dc.identifier.volume36
dc.identifier.wosWOS:000307699200006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Veterinary and Animal Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCarbonic anhydrase
dc.subjectCharacterization
dc.subjectHeat shock protein 70
dc.subjectHeavy metals
dc.subjectMuscle
dc.subjectRainbow trout
dc.subjectOncorhynchus mykiss
dc.subjectCarbonic anhydrase
dc.subjectCharacterization
dc.subjectHeat shock protein 70
dc.subjectHeavy metals
dc.subjectMuscle
dc.subjectRainbow trout
dc.subjectOncorhynchus mykiss
dc.titleChanges in carbonic anhydrase activity and gene expression of Hsp70 in rainbow trout (Oncorhynchus mykiss) muscle after exposure to some metalsen_US
dc.typeArticleen_US

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