4-aminothiophenol-intercalated montmorillonite: Organic-inorganic hybrid material as an immobilization support for biosensors

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dc.authoridTimur, Suna/0000-0002-1981-7577
dc.authoridOdaci Demirkol, Dilek/0000-0002-7954-1381
dc.authoridyalcinkaya, esra evrim/0000-0001-9376-0575
dc.contributor.authorYilmaz, Yahya Yasin
dc.contributor.authorYalcinkaya, Esra Evrim
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorTimur, Suna
dc.date.accessioned2024-10-04T18:48:06Z
dc.date.available2024-10-04T18:48:06Z
dc.date.issued2020
dc.departmentBayburt Üniversitesien_US
dc.description.abstractHybrid materials containing organic and inorganic structures are very important because they have the advantages of each of their components. Intercalation of the organic molecule to inorganic structures such as clay allows the preparation of multifunctional new immobilization matrices that contain both the strength properties of the clays and the properties of the organic molecule such as its functional groups (amine, carboxyl or hydroxyl etc.). In this study, 4-aminothiophenol intercalated montmorillonite (4ATP-Mt) was prepared and used as an immobilization layer for the pyranose oxidase (PyOx) enzyme on a glassy carbon (GC) electrode. Firstly, 4ATP intercalation of Mt was carried out, and the success of intercalation was proven using Fourier-Transform Infrared Spectroscopy (FTIR), X-ray Diffractometer (XRD), and Thermogravimetric Analysis (TGA) techniques. Then PyOx was immobilized using 4ATP-Mt, BSA and glutaraldehyde as a cross-linker on the surface of GC electrodes. To show the coating steps for the preparation of 4ATP-Mt/PyOx biosensors, cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques were applied, and surface morphology was visualized by scanning electron microscopy (SEM). After optimization of working conditions, analytical performance parameters were determined for glucose detection. The 4ATP-Mt/PyOx biosensor exhibited a wide linear concentration range between 0.01 and 0.5 mM (LOD: 1.0 mu M) for glucose. Additionally, the fabricated 4ATP-Mt/PyOx was assayed for the analysis of glucose in artificial body fluids and drinks.en_US
dc.description.sponsorshipEge University Scientific Research Projects Coordination Unit [16-FBE-023]en_US
dc.description.sponsorshipThis work was supported by Ege University Scientific Research Projects Coordination Unit. Project Number: 16-FBE-023.en_US
dc.identifier.doi10.1016/j.snb.2020.127665
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-85077458226en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.snb.2020.127665
dc.identifier.urihttp://hdl.handle.net/20.500.12403/2890
dc.identifier.volume307en_US
dc.identifier.wosWOS:000508110400003en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofSensors and Actuators B-Chemicalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject4-aminothiophenolen_US
dc.subjectMontmorilloniteen_US
dc.subjectPyranose oxidaseen_US
dc.subjectEnzymatic biosensoren_US
dc.title4-aminothiophenol-intercalated montmorillonite: Organic-inorganic hybrid material as an immobilization support for biosensorsen_US
dc.typeArticleen_US

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