The interactions of cephalosporins on polyol pathway enzymes from sheep kidney

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dc.authorid56711195000
dc.authorid6603903192
dc.contributor.authorŞengül B.
dc.contributor.authorBeydemir Ş.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:43:10Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:43:10Z
dc.date.issued2018
dc.departmentBayburt Üniversitesien_US
dc.description.abstractContext: Cephalosporins are derived from the fungus Acremonium. Due to their strong bactericidal ability, these drugs have to a wide usage in medicine. Objective: An investigation of the effects on sheep renal aldose reductase (AR) and sorbitol dehydrogenase (SDH) of cefoperazone, cefazolin, cefuroxime, ceftazidime and ceftriaxone as cephalosporin drugs was carried out in the present study. Methods: AR and SDH were purified from sheep kidney by ion exchange, gel filtration and affinity methods with approximately 219- and 484-fold, respectively. Some kinetic properties of the enzymes were determined such as optimal pH, optimal ionic strength, optimal temperature, stable pH, Km and Vmax. IC50 values of the drugs were found for each enzyme. Results: While the AR was inhibited by all drugs, SDH enzyme was inhibited by only CXM (IC50 8.10 mM). Interestingly, CZO activated SDH enzyme. This result was evaluated as important for the flow of the polyol reactions. Ki values and inhibition types were determined for AR. However, these values could not have determined for SDH, due to insufficient inhibition. Conclusions: From these results, it was concluded that cephalosporins may have an important effect on flow of the polyol metabolism. © 2017 Informa UK Limited, trading as Taylor & Francis Group.en_US
dc.identifier.doi10.1080/13813455.2017.1358749
dc.identifier.endpage44
dc.identifier.issn1381-3455
dc.identifier.issue1
dc.identifier.pmid28758816en_US
dc.identifier.scopus2-s2.0-85026521242en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage35
dc.identifier.urihttps://dx.doi.org/10.1080/13813455.2017.1358749
dc.identifier.urihttps://hdl.handle.net/20.500.12403/430
dc.identifier.volume124
dc.identifier.wosWOS:000425063500005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor and Francis Ltd
dc.relation.ispartofArchives of Physiology and Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAldose reductase
dc.subjectcephalosporins
dc.subjectdiabetes
dc.subjectinhibition
dc.subjectsorbitol dehydrogenase
dc.subjectaldehyde reductase
dc.subjectantiinfective agent
dc.subjectcefazolin
dc.subjectcefoperazone
dc.subjectceftazidime
dc.subjectceftriaxone
dc.subjectcefuroxime
dc.subjectcephalosporin derivative
dc.subjectenzyme inhibitor
dc.subjectglyceraldehyde
dc.subjectiditol dehydrogenase
dc.subjectsorbitol
dc.subjectanimal
dc.subjectantagonists and inhibitors
dc.subjectchemistry
dc.subjectcomparative study
dc.subjectdrug effect
dc.subjectenzyme activation
dc.subjectenzymology
dc.subjectisolation and purification
dc.subjectkidney
dc.subjectkinetics
dc.subjectmetabolism
dc.subjectmolecular model
dc.subjectpH
dc.subjectsheep
dc.subjecttemperature
dc.subjectAldehyde Reductase
dc.subjectAnimals
dc.subjectAnti-Bacterial Agents
dc.subjectCefazolin
dc.subjectCefoperazone
dc.subjectCeftazidime
dc.subjectCeftriaxone
dc.subjectCefuroxime
dc.subjectCephalosporins
dc.subjectEnzyme Activation
dc.subjectEnzyme Inhibitors
dc.subjectGlyceraldehyde
dc.subjectHydrogen-Ion Concentration
dc.subjectKidney
dc.subjectKinetics
dc.subjectL-Iditol 2-Dehydrogenase
dc.subjectModels, Molecular
dc.subjectSheep
dc.subjectSorbitol
dc.subjectTemperature
dc.subjectAldose reductase
dc.subjectcephalosporins
dc.subjectdiabetes
dc.subjectinhibition
dc.subjectsorbitol dehydrogenase
dc.subjectaldehyde reductase
dc.subjectantiinfective agent
dc.subjectcefazolin
dc.subjectcefoperazone
dc.subjectceftazidime
dc.subjectceftriaxone
dc.subjectcefuroxime
dc.subjectcephalosporin derivative
dc.subjectenzyme inhibitor
dc.subjectglyceraldehyde
dc.subjectiditol dehydrogenase
dc.subjectsorbitol
dc.subjectanimal
dc.subjectantagonists and inhibitors
dc.subjectchemistry
dc.subjectcomparative study
dc.subjectdrug effect
dc.subjectenzyme activation
dc.subjectenzymology
dc.subjectisolation and purification
dc.subjectkidney
dc.subjectkinetics
dc.subjectmetabolism
dc.subjectmolecular model
dc.subjectpH
dc.subjectsheep
dc.subjecttemperature
dc.subjectAldehyde Reductase
dc.subjectAnimals
dc.subjectAnti-Bacterial Agents
dc.subjectCefazolin
dc.subjectCefoperazone
dc.subjectCeftazidime
dc.subjectCeftriaxone
dc.subjectCefuroxime
dc.subjectCephalosporins
dc.subjectEnzyme Activation
dc.subjectEnzyme Inhibitors
dc.subjectGlyceraldehyde
dc.subjectHydrogen-Ion Concentration
dc.subjectKidney
dc.subjectKinetics
dc.subjectL-Iditol 2-Dehydrogenase
dc.subjectModels, Molecular
dc.subjectSheep
dc.subjectSorbitol
dc.subjectTemperature
dc.titleThe interactions of cephalosporins on polyol pathway enzymes from sheep kidneyen_US
dc.typeArticleen_US

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