Bioprospection of Metschnikowia sp. isolates as biocontrol agents against postharvest fungal decays on lemons with their potential modes of action

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dc.authoridKarbancioglu Guler, Funda/0000-0001-6576-0084
dc.authoridOztekin, Sebahat/0000-0003-0859-1776
dc.contributor.authorOztekin, Sebahat
dc.contributor.authorKarbancioglu-Guler, Funda
dc.date.accessioned2024-10-04T18:48:26Z
dc.date.available2024-10-04T18:48:26Z
dc.date.issued2021
dc.departmentBayburt Üniversitesien_US
dc.description.abstractIn this study, various fruit were employed as biocontrol yeast reservoirs, and eleven distinct yeast cultures of Metschnikowia sp. belonging to 6 different species were identified with sequence-based analysis of the D1/D2 domain of 26S rDNA. For initial screening, Metschnikowia isolates were tested on Fusarium oxysporum, Botrytis cinerea, Penicillium digitatum, Penicillium expansum, and Alternaria alternata. The highest antagonism was obtained on green and blue Penicillium (83.63-100 %). All tested yeasts showed chitinase activity, while some had protease, pectinase, cellulase, beta-1-3 glucanase, and gelatinase activities. Since lemons have high pectin content, three pectinase-free cultures at tested conditions with high in vitro antagonism on Penicillium were selected and used on lemons. The activities of the in vitro antifungal studies were found to be compatible with those of the in vivo, and P. digitatum's incidence was found to be higher than that of P. expansum on lemons. All tested pectinasefree Metschnikowia sp. lead to a significant reduction in the disease incidences and lesions at varying levels. The combined effect of lytic enzyme secretion, iron depletion, and volatile organic compounds (VOCs) production determined the antifungal mechanism of action. M. aff. fructicola demonstrated the highest biocontrol efficacy against Penicillium on lemons with an increasing shelf-life. The use of tentatively pectinase-negative Metschnikowia sp. as an antifungal biocontrol agent on lemons was considered for the first time. The findings will shed light on the effective use of Metschnikowia sp. as a potential biofungicide against the growth of postharvest fungal pathogens.en_US
dc.description.sponsorshipScientific Research Council of Istanbul Technical University [40878]en_US
dc.description.sponsorshipThis work was financially supported by the Scientific Research Council of Istanbul Technical University (grant no: 40878). We would like to thank Assoc. Prof. Dr. Enes Dertli (Department of Food Engineering, Yildiz Technical University, Istanbul, Turkey) for assistance in PCR analysis, and Prof. Dr. Nuh BOYRAZ (Department of Plant Protection, Selcuk University, Konya, Turkey) for kindly providing B. cinerea.en_US
dc.identifier.doi10.1016/j.postharvbio.2021.111634
dc.identifier.issn0925-5214
dc.identifier.issn1873-2356
dc.identifier.scopus2-s2.0-85108868595en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.postharvbio.2021.111634
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3052
dc.identifier.volume181en_US
dc.identifier.wosWOS:000692237800003en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofPostharvest Biology and Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiocontrol yeasten_US
dc.subjectMetschnikowia spen_US
dc.subjectBiofungicideen_US
dc.subjectPenicilliumen_US
dc.subjectPostharvest diseaseen_US
dc.subjectPulcherriminen_US
dc.titleBioprospection of Metschnikowia sp. isolates as biocontrol agents against postharvest fungal decays on lemons with their potential modes of actionen_US
dc.typeArticleen_US

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