Structural and bioactive characteristics of a dextran produced by Lactobacillus kunkeei AK1

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dc.authoridTaylan, Osman/0000-0002-5806-3237
dc.contributor.authorYilmaz, Mustafa Tahsin
dc.contributor.authorIspirli, Humeyra
dc.contributor.authorTaylan, Osman
dc.contributor.authorBilgrami, Anwar L.
dc.contributor.authorDertli, Enes
dc.date.accessioned2024-10-04T18:53:48Z
dc.date.available2024-10-04T18:53:48Z
dc.date.issued2022
dc.departmentBayburt Üniversitesien_US
dc.description.abstractIn this study, structural and techno-functional characteristics of an exopolysaccharide (EPS) produced by Lactobacillus kunkeei AK1 were determined. High-performance liquid chromatography (HPLC) analysis demonstrated that EPS AK1 was composed of only glucose units. H-1 and C-13 Nuclear magnetic resonance (NMR) analysis revealed that EPS AK1 was a dextran type EPS containing 4.78% (1 -> 4)-linked alpha-D-glucose branches. The molecular weight of EPS AK1 was determined to be 45 kDa by Gel Permeation Chromatography (GPC) analysis. A high level of thermal stability up to 280 degrees C was determined for dextran AK1 detected by Differential scanning calorimetry (DSC) and Thermogravimetric analysis (TGA). Dextran AK1 appeared as regular spheres with compact morphology and as irregular particles in the solution with no clear cross-linking between the chains of the polysaccharide observed by Scanning electron microscopy (SEM) and Atomic force microscopy (AFM) analysis, respectively. X-ray diffraction analysis (XRD) analysis demonstrated that dextran AK1 had a crystalline structure. A relatively strong antioxidant activity was observed for dextran AK1 determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging and cupric reducing antioxidant capacity (CUPRAC) tests. Finally, only a digestion ratio of 3.1% was observed for dextran AK1 following the in vitro simulated gastric digestion test.en_US
dc.description.sponsorshipDeanship of Scientific Research (DSR) at King Abdulaziz University, Jeddah, SaudiArabia [FP-60-42]en_US
dc.description.sponsorshipThe Deanship of Scientific Research (DSR) at King Abdulaziz University, Jeddah, SaudiArabia has funded this project, under grant no. FP-60-42. The authors, therefore, gratefully acknowledge the DSR technical and financial support.en_US
dc.identifier.doi10.1016/j.ijbiomac.2022.01.012
dc.identifier.endpage302en_US
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid35016972en_US
dc.identifier.scopus2-s2.0-85122626896en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage293en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2022.01.012
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3702
dc.identifier.volume200en_US
dc.identifier.wosWOS:000797931800004en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLactobacillus kunkeeien_US
dc.subjectDextranen_US
dc.subjectLABen_US
dc.subjectExopolysaccharidesen_US
dc.subjectStructural and bioactive characteristicsen_US
dc.titleStructural and bioactive characteristics of a dextran produced by Lactobacillus kunkeei AK1en_US
dc.typeArticleen_US

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