Effects of processing methods and extraction solvents on the chemical content and bioactive properties of propolis

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dc.authoridBayram, Sinan/0000-0002-2156-1566
dc.authoridGERCEK, YUSUF CAN/0000-0001-5372-0229
dc.contributor.authorBayram, Nesrin Ecem
dc.contributor.authorGercek, Yusuf Can
dc.contributor.authorBayram, Sinan
dc.contributor.authorTogar, Basak
dc.date.accessioned2024-10-04T18:49:39Z
dc.date.available2024-10-04T18:49:39Z
dc.date.issued2020
dc.departmentBayburt Üniversitesien_US
dc.description.abstractThe aim of this study was to evaluate the bioactive properties of propolis extract prepared using different solvents and different extraction methods. The extracts were analyzed by gas chromatography-mass spectrometry (GC-MS) and the differences between their antibacterial activities were evaluated by disc diffusion method. At the same time, bioactive properties of different concentrations of propolis extracts were investigated on human umbilical vein endothelial cells (HUVEC). The proliferative effects and cytotoxic effects of the extracts were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) analyzes, respectively. Total antioxidant capacity (TAC) and total oxidative status (TOS) parameters were used in assessing biochemical effects in the HUVEC cell line. The DNA damage was also analyzed by 8-oxo-2-deoxyguanosine (8-OHdG) level as indicators of genotoxicity. As a result of the MTT analysis conducted within the scope of the present study, the extracts tested were sorted as 95% ethanol extract of propolis (PEE95) > ultrasonic ethanol extract of propolis (PUEE) > 50% ethanol extract of propolis (PEE50) > ultrasonic water extract of propolis (PUWE) in terms of the effectiveness of their cell viabilities. It was observed that high concentrations of PEE95 induced LDH release. In addition to this, our findings have shown that PEE50, PUEE and PUWE increased oxidative stress at high concentrations. According to 8-OH-dG analysis, all tested extracts were found to be non-genotoxic. The results obtained from antibacterial activity and minimum inhibition concentration tests showed that PUEE and PEE95 had stronger antibacterial effects than PEE50 and PUWE. All these results indicated that propolis has beneficial effects for human health and therefore it is a valuable product which can be used as a food supplement.en_US
dc.identifier.doi10.1007/s11694-019-00340-z
dc.identifier.endpage916en_US
dc.identifier.issn2193-4126
dc.identifier.issn2193-4134
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85076302949en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage905en_US
dc.identifier.urihttps://doi.org/10.1007/s11694-019-00340-z
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3246
dc.identifier.volume14en_US
dc.identifier.wosWOS:000520002400028en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Food Measurement and Characterizationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPropolisen_US
dc.subjectCytotoxicityen_US
dc.subjectAntioxidant capacityen_US
dc.subjectOxidative stressen_US
dc.subjectGenotoxicityen_US
dc.titleEffects of processing methods and extraction solvents on the chemical content and bioactive properties of propolisen_US
dc.typeArticleen_US

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