Investigation of CYP2E1 and Caspase-3 Gene Expressions in COVID-19 patients

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dc.contributor.authorUzuncakmak, S. Karabulut
dc.contributor.authorDirican, E.
dc.contributor.authorNaldan, M. E.
dc.contributor.authorCan, F. Kesmez
dc.contributor.authorHallcl, Z.
dc.date.accessioned2024-10-04T18:51:11Z
dc.date.available2024-10-04T18:51:11Z
dc.date.issued2022
dc.departmentBayburt Üniversitesien_US
dc.description.abstractBackground: COVID-19 pandemic spread around the world like an infectious disease that presents waved effects on patients. Some patients needed ICU and respiratory support. Some patients only had flu-like symptoms. Cytokine storm and elevated ROS were serious problems for treatment. Apoptotic genes and CYP Family are part of these mechanisms. Aim: In this study, our aim was to examine the gene expression CYP2E1 and Caspase-3 in patients with COVID-19 infection. Method: 60 COVID-19(+) patients (ICU and non-ICU patients) and 30 healthy volunteers were enrolled to study. To measure the level of gene expression qPCR was used. The 2-Delta Delta Ct method was utilized to analyze gene expression. Results: The expression of CYP2E1 and Caspase-3 genes showed a significant discrepancy between patients and healthy individuals. Caspase-3 expression increased (p=0,0041) but CYP2E1 expression decreased (p=0,0214) in COVID-19 patients compared to healthy individuals. Both levels of gene expression were lower in patients with affected lungs than patients with unaffected lungs (p<0,05). Laboratory findings including d-Dimer, LDH, platelet count, lymphocyte count were related to both gene expressions (p<0,05). We found no correlation between CYP2E1 and Caspase-3 expressions. Conclusion: The expression of Caspase-3 demonstrated apoptotic situations of patients but was not related to the CYP2E1 expression level. CYP2E1 gene expression is an important actor to metabolize endogens and xenobiotics however, COVID-19 patients demonstrated decreased CYP2E1 expression. CYP2E1 and Caspase-3 gene expression levels may be used as a diagnostic tool for COVID-19 patients.en_US
dc.identifier.doi10.1016/j.genrep.2022.101497
dc.identifier.issn2452-0144
dc.identifier.pmid35071821en_US
dc.identifier.scopus2-s2.0-85122962408en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.urihttps://doi.org/10.1016/j.genrep.2022.101497
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3417
dc.identifier.volume26en_US
dc.identifier.wosWOS:000745658300003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofGene Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCCYP2E1en_US
dc.subjectCOVID-19en_US
dc.subjectCaspase-3en_US
dc.subjectSARS-CoV-2en_US
dc.titleInvestigation of CYP2E1 and Caspase-3 Gene Expressions in COVID-19 patientsen_US
dc.typeArticleen_US

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