Structure and biosynthesis of two exopolysaccharides produced by lactobacillus johnsonii FI9785

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dc.authorid36815706500
dc.authorid7007143861
dc.authorid7005140433
dc.authorid8339792100
dc.authorid7003264364
dc.authorid57194742676
dc.authorid7402464998
dc.authorid6603867601
dc.contributor.authorDertli E.
dc.contributor.authorColquhoun I.J.
dc.contributor.authorGunning A.P.
dc.contributor.authorBongaerts R.J.
dc.contributor.authorLe Gall G.
dc.contributor.authorBonev B.B.
dc.contributor.authorMayer M.J.
dc.contributor.authorNarbad A.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:44:21Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:44:21Z
dc.date.issued2013
dc.departmentBayburt Üniversitesien_US
dc.description.abstractExopolysaccharides were isolated and purified from Lactobacillus johnsonii FI9785, which has previously been shown to act as a competitive exclusion agent to control Clostridium perfringens in poultry. Structural analysis by NMR spectroscopy revealed that L. johnsonii FI9785 can produce two types of exopolysaccharide: EPS-1 is a branched dextran with the unusual feature that every backbone residue is substituted with a 2-linked glucose unit, and EPS-2 was shown to have a repeating unit with the following structure: -6)-?-Glcp-(1-3)-?-Glcp-(1-5)-?-Galf-(1-6)-?-Glcp-(1-4) -?-Gal p-(1-4)-?-Glcp-(1-. Sites on both polysaccharides were partially occupied by substituent groups: 1-phosphoglycerol and O-acetyl groups in EPS-1 and a single O-acetyl group in EPS-2. Analysis of a deletion mutant (?epsE) lacking the putative priming glycosyltransferase gene located within a predicted eps gene cluster revealed that the mutant could produce EPS-1 but not EPS-2, indicating that epsE is essential for the biosynthesis of EPS-2. Atomic force microscopy confirmed the localization of galactose residues on the exterior of wild type cells and their absence in the ?epsE mutant. EPS2 was found to adopt a random coil structural conformation. Deletion of the entire 14-kb eps cluster resulted in an acapsular mutant phenotype that was not able to produce either EPS-2 or EPS-1. Alterations in the cell surface properties of the EPS-specific mutants were demonstrated by differences in binding of an anti-wild type L. johnsonii antibody. These findings provide insights into the biosynthesis and structures of novel exopolysaccharides produced by L. johnsonii FI9785, which are likely to play an important role in biofilm formation, protection against harsh environment of the gut, and colonization of the host. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.en_US
dc.identifier.doi10.1074/jbc.M113.507418
dc.identifier.endpage31951
dc.identifier.issn0021-9258
dc.identifier.issue44
dc.identifier.pmid24019531en_US
dc.identifier.scopus2-s2.0-84887093174en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage31938
dc.identifier.urihttps://dx.doi.org/10.1074/jbc.M113.507418
dc.identifier.urihttps://hdl.handle.net/20.500.12403/835
dc.identifier.volume288
dc.identifier.wosWOS:000330596200046en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiofilm formation
dc.subjectCell surface properties
dc.subjectClostridium perfringens
dc.subjectExopolysaccharides
dc.subjectGalactose residues
dc.subjectHarsh environment
dc.subjectPriming glycosyltransferase genes
dc.subjectStructural conformations
dc.subjectAtomic force microscopy
dc.subjectBiochemistry
dc.subjectBiosynthesis
dc.subjectCell membranes
dc.subjectNuclear magnetic resonance spectroscopy
dc.subjectPolysaccharides
dc.subjectMetabolites
dc.subjectbacterium antibody
dc.subjectexopolysaccharide
dc.subjectgalactose
dc.subjectglycosyltransferase
dc.subjectarticle
dc.subjectatomic force microscopy
dc.subjectbacterial strain
dc.subjectbacterium culture
dc.subjectbiosynthesis
dc.subjectcarbohydrate analysis
dc.subjectconformation
dc.subjectcontrolled study
dc.subjectflow cytometry
dc.subjectgene cluster
dc.subjectLactobacillus johnsonii
dc.subjectLactobacillus johnsonii FI9785
dc.subjectmutant
dc.subjectnonhuman
dc.subjectnuclear magnetic resonance spectroscopy
dc.subjectphenotype
dc.subjectpriority journal
dc.subjecttransmission electron microscopy
dc.subjectwild type
dc.subjectAtomic Force Microscopy
dc.subjectBacteria
dc.subjectCarbohydrate Structure
dc.subjecteps Cluster
dc.subjectExopolysaccharide
dc.subjectLactobacillus johnsonii
dc.subjectMutant
dc.subjectNuclear Magnetic Resonance
dc.subjectBacterial Proteins
dc.subjectCarbohydrate Conformation
dc.subjectGenes, Bacterial
dc.subjectGlucosyltransferases
dc.subjectLactobacillus
dc.subjectMultigene Family
dc.subjectMutation
dc.subjectPolysaccharides, Bacterial
dc.subjectBiofilm formation
dc.subjectCell surface properties
dc.subjectClostridium perfringens
dc.subjectExopolysaccharides
dc.subjectGalactose residues
dc.subjectHarsh environment
dc.subjectPriming glycosyltransferase genes
dc.subjectStructural conformations
dc.subjectAtomic force microscopy
dc.subjectBiochemistry
dc.subjectBiosynthesis
dc.subjectCell membranes
dc.subjectNuclear magnetic resonance spectroscopy
dc.subjectPolysaccharides
dc.subjectMetabolites
dc.subjectbacterium antibody
dc.subjectexopolysaccharide
dc.subjectgalactose
dc.subjectglycosyltransferase
dc.subjectarticle
dc.subjectatomic force microscopy
dc.subjectbacterial strain
dc.subjectbacterium culture
dc.subjectbiosynthesis
dc.subjectcarbohydrate analysis
dc.subjectconformation
dc.subjectcontrolled study
dc.subjectflow cytometry
dc.subjectgene cluster
dc.subjectLactobacillus johnsonii
dc.subjectLactobacillus johnsonii FI9785
dc.subjectmutant
dc.subjectnonhuman
dc.subjectnuclear magnetic resonance spectroscopy
dc.subjectphenotype
dc.subjectpriority journal
dc.subjecttransmission electron microscopy
dc.subjectwild type
dc.subjectAtomic Force Microscopy
dc.subjectBacteria
dc.subjectCarbohydrate Structure
dc.subjecteps Cluster
dc.subjectExopolysaccharide
dc.subjectLactobacillus johnsonii
dc.subjectMutant
dc.subjectNuclear Magnetic Resonance
dc.subjectBacterial Proteins
dc.subjectCarbohydrate Conformation
dc.subjectGenes, Bacterial
dc.subjectGlucosyltransferases
dc.subjectLactobacillus
dc.subjectMultigene Family
dc.subjectMutation
dc.subjectPolysaccharides, Bacterial
dc.titleStructure and biosynthesis of two exopolysaccharides produced by lactobacillus johnsonii FI9785en_US
dc.typeArticleen_US

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