Screening for antioxidant capacity, pollen types and phytochemical profile by GC/MS and UHPLC from propolis

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dc.authoridGERCEK, YUSUF CAN/0000-0001-5372-0229
dc.contributor.authorBayram, Nesrin Ecem
dc.contributor.authorGercek, Yusuf Can
dc.contributor.authorOz, Gul Cevahir
dc.date.accessioned2024-10-04T18:52:51Z
dc.date.available2024-10-04T18:52:51Z
dc.date.issued2020
dc.departmentBayburt Üniversitesien_US
dc.description.abstractPropolis is a resinous mixture collected by honeybees from different parts of plants such as poplars, birches, alders, conifers, pines, palms and willows. This study aims to determine pollen types (by light microscope), chemical compound profile (by GC-MS), fatty acid composition (by GC-MS), total phenolic content (by the Folin-Ciocalteu method), total flavonoid content (by the aluminium chloride method) and antioxidant capacity (by the CUPRAC, ABTS and CERAC methods) of a propolis sample from the western part of Istanbul, Turkey. As a result of microscopic analysis of the sample, pollen types of taxa belonging to 27 plant families were diagnosed. The GC-MS analysis of propolis revealed the presence of 38 phytochemical constituents that may contribute to its quality. Of these compounds, rates of 4H-1-benzopyran-4-one, 2,3-dihydro-5,7-dihydroxy-2-phenyl-,(S) were highest. In addition, the concentrations of naringenin, pinocembrin and galangin were determined to be 2.45, 9.92 and 7.06 mg/ml by UHPLC analysis. The extract had significant antioxidant activity in all assays, with values of 282.8 +/- 9 mg TE/g in the CUPRAC, 425.7 +/- 18 mg TE/g in the CERAC and 186.4 +/- 8 mg TE/g in the ABTS assays. Antioxidant capacity of the propolis extract was positively associated with the total phenolic and flavonoid contents of the extract. Moreover, the major fatty acids were C20:1n9 (cis-11-eicosenoic acid), C22:1 (erucic acid) and C24:1 (nervonic acid). The results show that the propolis used in our study has important potential as an alternative food supplement and for cosmetic and therapeutic medicine and it can be used as an active agent in these areas.en_US
dc.description.sponsorshipIstanbul University [26966]en_US
dc.description.sponsorshipThis study was supported by Scientific Research Projects Unit (Project code: 26966) from Istanbul University.en_US
dc.identifier.doi10.23751/pn.v22i3.8416
dc.identifier.issn1129-8723
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85095456802en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.urihttps://doi.org/10.23751/pn.v22i3.8416
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3670
dc.identifier.volume22en_US
dc.identifier.wosWOS:000583879700019en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMattioli 1885en_US
dc.relation.ispartofProgress in Nutritionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPropolisen_US
dc.subjectpollen content of propolisen_US
dc.subjectfatty acid of propolisen_US
dc.subjectantioxidant activityen_US
dc.titleScreening for antioxidant capacity, pollen types and phytochemical profile by GC/MS and UHPLC from propolisen_US
dc.typeArticleen_US

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