The toxicological impacts of some heavy metals on carbonic anhydrase from gilthead sea bream (Sparus aurata) gills
Küçük Resim Yok
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
It is known that heavy metals have toxic effects on fish. Insufficient measures are a serious problem in our country and around the world. This problem can threaten human health in areas where it is common for people to obtain nutrition from local bodies of water. In this study, the toxicological impacts of some heavy metals were investigated on carbonic anhydrase activity in gilthead gills. Carbonic anhydrase (CA) was purified from gilthead sea bream (Sparus aurata) gills with a specific activity of 2872.92EUmg-1 and a yield of 32.84% using affinity chromatography. The overall purification was approximately ~84-fold. SDS-polyacrylamide gel electrophoresis showed a single band, and the MW was approximately 30.5kDa (Soyut et al., 2008, 2012; Soyut and Beydemir, 2008, 2012; Kaya et al., 2013). The kinetic and characteristic properties of CA such as the optimum pH, stable pH, optimum temperature, activation energy (Ea), activation enthalpy (?H), Q10, Km and Vmax were determined. Cadmium (Cd2+), copper (Cu2+), nickel (Ni2+) and silver (Ag+) inhibited CA activity in in vitro conditions. Ki values were calculated for these metals. Ki values were 31.20mM for cadmium (Cd2+), 161.96mM for copper (Cu2+), 10.79mM for nickel (Ni2+) and 0.0082mM for silver (Ag+) based on Lineweaver-Burk plots. Except for cadmium, heavy metals had the same inhibition mechanism. Cadmium was competitive, and the others were noncompetitive. © 2015 Elsevier B.V.
Açıklama
Anahtar Kelimeler
Carbonic anhydrase, Characterization, Gills, Gilthead sea bream (Sparus aurata), Heavy metals, cadmium, carbonate dehydratase, copper, dodecyl sulfate sodium, heavy metal, nickel, silver, carbonate dehydratase, heavy metal, water pollutant, affinity chromatography, animal cell, animal tissue, Article, concentration (parameters), contaminated fish, controlled study, enthalpy, environmental impact assessment, enzyme activation, enzyme activity, enzyme assay, enzyme inhibition, enzyme kinetics, enzyme purification, enzyme stability, gill, in vitro study, molecular weight, nonhuman, pH, polyacrylamide gel electrophoresis, priority journal, Sparus aurata, suicide substrate, toxicology, animal, drug effects, enzymology, metabolism, sea bream, toxicity, water pollutant, Archosargus rhomboidalis, Sparus aurata, Torreya nucifera, Animals, Carbonic Anhydrases, Gills, Metals, Heavy, Sea Bream, Water Pollutants, Chemical, Carbonic anhydrase, Characterization, Gills, Gilthead sea bream (Sparus aurata), Heavy metals, cadmium, carbonate dehydratase, copper, dodecyl sulfate sodium, heavy metal, nickel, silver, carbonate dehydratase, heavy metal, water pollutant, affinity chromatography, animal cell, animal tissue, Article, concentration (parameters), contaminated fish, controlled study, enthalpy, environmental impact assessment, enzyme activation, enzyme activity, enzyme assay, enzyme inhibition, enzyme kinetics, enzyme purification, enzyme stability, gill, in vitro study, molecular weight, nonhuman, pH, polyacrylamide gel electrophoresis, priority journal, Sparus aurata, suicide substrate, toxicology, animal, drug effects, enzymology, metabolism, sea bream, toxicity, water pollutant, Archosargus rhomboidalis, Sparus aurata, Torreya nucifera, Animals, Carbonic Anhydrases, Gills, Metals, Heavy, Sea Bream, Water Pollutants, Chemical
Kaynak
Environmental Toxicology and Pharmacology
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
39
Sayı
2
