EpsA is an essential gene in exopolysaccharide production in Lactobacillus johnsonii FI9785

Basit Öğe Kaydı
dc.authorid36815706500
dc.authorid7402464998
dc.authorid7007143861
dc.authorid6603867601
dc.contributor.authorDertli E.
dc.contributor.authorMayer M.J.
dc.contributor.authorColquhoun I.J.
dc.contributor.authorNarbad A.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:43:35Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:43:35Z
dc.date.issued2016
dc.departmentBayburt Üniversitesien_US
dc.description.abstractLactobacillus johnsonii FI9785 has an eps gene cluster which is required for the biosynthesis of homopolymeric exopolysaccharides (EPS)-1 and heteropolymeric EPS-2 as a capsular layer. The first gene of the cluster, epsA, is the putative transcriptional regulator. In this study we showed the crucial role of epsA in EPS biosynthesis by demonstrating that deletion of epsA resulted in complete loss of both EPS-1 and EPS-2 on the cell surface. Plasmid complementation of the epsA gene fully restored EPS production, as confirmed by transmission electron microscopy and nuclear magnetic resonance (NMR) analysis. Furthermore, this complementation resulted in a twofold increase in the expression levels of this gene, which almost doubled amounts of EPS production in comparison with the wild-type strain. Analysis of EPS by NMR showed an increased ratio of the heteropolysaccharide to homopolysaccharide in the complemented strain and allowed identification of the acetylated residue in EPS-2 as the (1,4)-linked ?Glcp unit, with the acetyl group located at O-6. These findings indicate that epsA is a positive regulator of EPS production and that EPS production can be manipulated by altering its expression. © 2015 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.en_US
dc.identifier.doi10.1111/1751-7915.12314
dc.identifier.endpage501
dc.identifier.issn1751-7907
dc.identifier.issue4
dc.identifier.pmid26401596en_US
dc.identifier.scopus2-s2.0-84976557103en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage496
dc.identifier.urihttps://dx.doi.org/10.1111/1751-7915.12314
dc.identifier.urihttps://hdl.handle.net/20.500.12403/607
dc.identifier.volume9
dc.identifier.wosWOS:000379689900008en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherJohn Wiley and Sons Ltd
dc.relation.ispartofMicrobial Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectexopolysaccharide
dc.subjectArticle
dc.subjectbacterial gene
dc.subjectbacterial strain
dc.subjectbiosynthesis
dc.subjectcell surface
dc.subjectcontrolled study
dc.subjectEpsA gene
dc.subjectgene deletion
dc.subjectgene expression
dc.subjectgene function
dc.subjectLactobacillus johnsonii
dc.subjectnonhuman
dc.subjectnuclear magnetic resonance
dc.subjectplasmid
dc.subjecttransmission electron microscopy
dc.subjectexopolysaccharide
dc.subjectArticle
dc.subjectbacterial gene
dc.subjectbacterial strain
dc.subjectbiosynthesis
dc.subjectcell surface
dc.subjectcontrolled study
dc.subjectEpsA gene
dc.subjectgene deletion
dc.subjectgene expression
dc.subjectgene function
dc.subjectLactobacillus johnsonii
dc.subjectnonhuman
dc.subjectnuclear magnetic resonance
dc.subjectplasmid
dc.subjecttransmission electron microscopy
dc.titleEpsA is an essential gene in exopolysaccharide production in Lactobacillus johnsonii FI9785en_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu