Applicability of Phenolic Profile Analysis Method Developed with RP-HPLC-PDA to some Bee Product

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dc.authoridKARA, Yakup/0000-0003-3121-5023
dc.contributor.authorKara, Yakup
dc.contributor.authorCan, Zehra
dc.contributor.authorKolayl, Sevgi
dc.date.accessioned2024-10-04T18:48:17Z
dc.date.available2024-10-04T18:48:17Z
dc.date.issued2022
dc.departmentBayburt Üniversitesien_US
dc.description.abstractIn this work, a reverse phase high-performance liquid chromatographic method (RP-HPLC) with photo diode array (PDA) at 4 different wavelengths (250, 280, 320 and 360) for the determination of some polyphenols in some bee products (honey, pollen, and propolis) is developed. The analyses were carried out on C18 column (250 mm x 4.6 mm, 5 mu m; GL Sciences), and the gradient program was used with a mobile phase A reservoir with 70% acetonitrile and the B reservoir with 2% acetic acid. The HPLC method was founded that limit of detection was in the range 0.022-0.0908 mu g/mL; the limit of quantification was in the range 0.074-0.3027 mu g/mL, all calibration curves were linear R-2>0.995 within the range, the recovery range was 91.43- 111.37% for 10 ppm and 98.44-101.68% for 40 ppm and relative error levels 0.0330-0.0290 respectively. The developed method was applied to some bee products available on the Turkey market. The study aimed at phenolic profiles of the bee products extracts were revealed by using 25 phenolic standards. The proposed method was optimized, quickly, and simple validated by evaluating the linear range, the limits of detection and quantification, the accuracy, the precious, the repeatability, and recoveries suitable for the phenolic analysis. It is concluded that evaluated and the quantitative determination of the bee products can be made quickly and reliably with the optimized method.en_US
dc.identifier.doi10.1590/1678-4324-2022210384
dc.identifier.issn1516-8913
dc.identifier.issn1678-4324
dc.identifier.scopus2-s2.0-85141124798en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1590/1678-4324-2022210384
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3005
dc.identifier.volume65en_US
dc.identifier.wosWOS:000855790400001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInst Tecnologia Paranaen_US
dc.relation.ispartofBrazilian Archives of Biology and Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectValidationen_US
dc.subjectoptimizationen_US
dc.subjectHPLC-PDAen_US
dc.subjectphenolicen_US
dc.subjectflavonoidsen_US
dc.subjectbee productsen_US
dc.titleApplicability of Phenolic Profile Analysis Method Developed with RP-HPLC-PDA to some Bee Producten_US
dc.typeArticleen_US

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