Optimization of lactose derivative hetero-oligosaccharides production using whey as the acceptor molecule by an active glucansucrase

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dc.authoridTaylan, Osman/0000-0002-5806-3237
dc.authoridBalubaid, Mohammed/0000-0001-7978-401X
dc.contributor.authorKabli, Mohammad
dc.contributor.authorIspirli, Humeyra
dc.contributor.authorBalubaid, Mohammed
dc.contributor.authorTaylan, Osman
dc.contributor.authorYilmaz, Mustafa Tahsin
dc.contributor.authorDertli, Enes
dc.date.accessioned2024-10-04T18:52:33Z
dc.date.available2024-10-04T18:52:33Z
dc.date.issued2022
dc.departmentBayburt Üniversitesien_US
dc.description.abstractGlucansucrases are capable of synthesis of distinct oligosaccharides using different acceptor molecules and in this study lactose derivative hetero-oligosaccharides were shown to be produced by an active glucansucrase in its acceptor reaction with sucrose and whey as the donor-acceptor molecules, respectively. Following the monitoring of the reaction products by TLC analysis, the effects of reaction parameters in terms of enzyme concentration, sucrose/whey ratio and reaction temperature on final oligosaccharide yields (OY) were optimized by Response Surface Methodology (RSM). Results indicated that increment of enzyme concentration and reaction temperature decreased the OY in a particular reaction time whereas the OY increased with the increment of sucrose/whey ratio in the acceptor reactions of glucansucrase E81 with whey as the acceptor molecule. A low level of glucansucrase E81 concentration was sufficient to obtain the highest OY in the tested conditions. These findings demonstrated the importance of the optimisation of the reaction parameters for the acceptor reactions of the glucansucrases.en_US
dc.description.sponsorshipDeanship of Scientific Research (DSR) at King Abdulaziz University, Jeddah [RG-39-130-18]; DSRen_US
dc.description.sponsorshipThis project was funded by the Deanship of Scientific Research (DSR) at King Abdulaziz University, Jeddah, under grant no. (RG-39-130-18). The authors, therefore, acknowledge with thanks DSR technical and financial support.en_US
dc.identifier.doi10.1080/10242422.2020.1828372
dc.identifier.endpage16en_US
dc.identifier.issn1024-2422
dc.identifier.issn1029-2446
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85091798308en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage9en_US
dc.identifier.urihttps://doi.org/10.1080/10242422.2020.1828372
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3551
dc.identifier.volume40en_US
dc.identifier.wosWOS:000573651000001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofBiocatalysis and Biotransformationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlucansucrasesen_US
dc.subjectacceptor reactionsen_US
dc.subjectwheyen_US
dc.subjectRSMen_US
dc.subjectlactose derivative hetero-oligosaccharidesen_US
dc.subjectoligosaccharide yielden_US
dc.titleOptimization of lactose derivative hetero-oligosaccharides production using whey as the acceptor molecule by an active glucansucraseen_US
dc.typeArticleen_US

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