Structural analysis of the ?-D-glucan produced by the sourdough isolate Lactobacillus brevis E25
Küçük Resim Yok
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Cereal-associated Lactic Acid Bacteria (LAB) are well known for homopolymeric exopolysaccharide (EPS) production. Herein, the structure of an EPS isolated from sourdough isolate Lactobacillus brevis E25 was determined. A modified BHI medium was used for production of EPS-E25 in order to eliminate potential contaminants. Analysis of sugar monomers in EPS revealed that glucose was the only sugar present. Structural characterisation of EPS by NMR and methylation analysis revealed that E25 produced a highly branched ?-glucan with (?1 ? 3) and (?1 ? 6) glycosidic linkages, and was similar in structure to a previously reported EPS from Lactobacillus reuteri 180. The 1H and 13C NMR data were contrasted with newly recorded data for known polysaccharides (alternan, commercial dextran) which also contain ?-(1,3,6)Glc branch points. It was found in both E25 EPS and alternan that NMR parameters could be used to distinguish glucose residues that had the same substitution pattern but occupied different positions in the structure. © 2017 Elsevier Ltd
Açıklama
Anahtar Kelimeler
Exopolysaccharides, Lactobacillus brevis, NMR analysis, ?-D-Glucan, Alkylation, Glucose, Lactic acid, Metabolites, D-glucan, Exopolysaccharides, Lactobacillus brevis, Lactobacillus reuteri, Methylation analysis, NMR analysis, Potential contaminants, Substitution patterns, Polysaccharides, alpha dextro glucan, bacterial polysaccharide, dextran, exopolysaccharide, glucan, glucose, unclassified drug, alternan, bacterial polysaccharide, glucan, Article, bacterial strain, bacterium culture, bacterium isolate, carbon nuclear magnetic resonance, chemical structure, Lactobacillus brevis, Leuconostoc mesenteroides, methylation, proton nuclear magnetic resonance, chemistry, food grain, Lactobacillus brevis, Lactobacillus reuteri, nuclear magnetic resonance spectroscopy, Dextrans, Edible Grain, Glucans, Lactobacillus brevis, Lactobacillus reuteri, Magnetic Resonance Spectroscopy, Polysaccharides, Bacterial, Exopolysaccharides, Lactobacillus brevis, NMR analysis, ?-D-Glucan, Alkylation, Glucose, Lactic acid, Metabolites, D-glucan, Exopolysaccharides, Lactobacillus brevis, Lactobacillus reuteri, Methylation analysis, NMR analysis, Potential contaminants, Substitution patterns, Polysaccharides, alpha dextro glucan, bacterial polysaccharide, dextran, exopolysaccharide, glucan, glucose, unclassified drug, alternan, bacterial polysaccharide, glucan, Article, bacterial strain, bacterium culture, bacterium isolate, carbon nuclear magnetic resonance, chemical structure, Lactobacillus brevis, Leuconostoc mesenteroides, methylation, proton nuclear magnetic resonance, chemistry, food grain, Lactobacillus brevis, Lactobacillus reuteri, nuclear magnetic resonance spectroscopy, Dextrans, Edible Grain, Glucans, Lactobacillus brevis, Lactobacillus reuteri, Magnetic Resonance Spectroscopy, Polysaccharides, Bacterial
Kaynak
Food Chemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
242
