Determination of toxicity and radioprotective properties of bacterial and fungal eumelanin pigments

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dc.authoridBayram, Sinan/0000-0002-2156-1566
dc.authoridKarabulut, Abdulhalik/0000-0003-2290-9007
dc.authoridAygun, Bunyamin/0000-0002-9384-1540
dc.authoridGulluce, Medine/0000-0002-5957-8259
dc.contributor.authorBayram, Sinan
dc.contributor.authorAygun, Bunyamin
dc.contributor.authorKaradayi, Mehmet
dc.contributor.authorAlaylar, Burak
dc.contributor.authorGulluce, Medine
dc.contributor.authorKarabulut, Abdulhalik
dc.date.accessioned2024-10-04T18:49:28Z
dc.date.available2024-10-04T18:49:28Z
dc.date.issued2023
dc.departmentBayburt Üniversitesien_US
dc.description.abstractPurposeDetermination of the protective property of melanin, an organic polymer class consisting of phenolic and/or indolic compounds isolated from bacteria and fungi, against fast neutron radiation. To show that these melanin samples, which also have antioxidant and metal chelating properties, can be used as an active ingredient for a drug to be developed against neutrons used in nuclear research and medicine.Materials and methodsBacterial and fungal media were prepared, and melanin pigments were produced and isolated. For molecular characterization of pigments, bacterial genomic DNA extraction, 16S rDNA gene amplification processes, and fungal genomic DNA extraction, ITS1, and ITS4 Gene Regions amplification were performed. The DEL assay was implemented to determine the genotoxicity properties of bacterial and fungal melanin pigments. Samples were prepared in a pad measuring 10 ml volume (60 x 15 mm) at a concentration of 0.2-1 microgram in 1% agarose gel for radiation-absorbed dose measurements. Absorption measurements were made using Am-241-Be fast neutron source and Canberra brand NP series BF3 gaseous detector to determine the neutron radiation absorption capacity of all samples. The results obtained to determine the absorption degrees of melanin samples were compared with paraffin and normal concrete, which are widely used in neutron radiation shielding studies.ResultsMelanin pigments were obtained using different bacteria and fungi strains. Afterwards, the fast neutron radiation absorption capacity of these purified pigments were determined. Compared to reference samples, these pigments were found to have slightly lower radiation absorbing ability. In addition to these experiments, cytotoxicity tests were carried out using the Yeast DEL assay technique to evaluate the potential for use of these organic pigments in fields such as medicine and pharmacology. According to the results obtained from the tests, it was determined that these melanin samples did not have any toxic effects.ConclusionIt was determined that these melanin samples have the potential to be used as a radioprotective drug active substance to protect the tissues and cells of people exposed to neutron radiation after a nuclear accident or nuclear war.Giving a drug that will be developed by using these active ingredients before or after people are exposed to a radiation environment can provide great benefits.en_US
dc.identifier.doi10.1080/09553002.2023.2204957
dc.identifier.endpage1793en_US
dc.identifier.issn0955-3002
dc.identifier.issn1362-3095
dc.identifier.issue11en_US
dc.identifier.pmid37071465en_US
dc.identifier.scopus2-s2.0-85158854565en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1785en_US
dc.identifier.urihttps://doi.org/10.1080/09553002.2023.2204957
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3168
dc.identifier.volume99en_US
dc.identifier.wosWOS:000975935700001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofInternational Journal of Radiation Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMelaninen_US
dc.subjectneutron shieldingen_US
dc.subjectradiation shieldingen_US
dc.subjectyeast DEL assayen_US
dc.titleDetermination of toxicity and radioprotective properties of bacterial and fungal eumelanin pigmentsen_US
dc.typeArticleen_US

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