Bioproduction, structure elucidation and in vitro antiproliferative effect of eumelanin pigment fromStreptomyces parvusBSB49

Basit Öğe Kaydı
dc.authoridGERCEK, YUSUF CAN/0000-0001-5372-0229
dc.authoridBayram, Sinan/0000-0002-2156-1566
dc.authoridDengiz, Cagatay/0000-0002-8238-6941
dc.contributor.authorBayram, Sinan
dc.contributor.authorDengiz, Cagatay
dc.contributor.authorGercek, Yusuf Can
dc.contributor.authorCetin, Idil
dc.contributor.authorTopcul, Mehmet R.
dc.date.accessioned2024-10-04T18:48:25Z
dc.date.available2024-10-04T18:48:25Z
dc.date.issued2020
dc.departmentBayburt Üniversitesien_US
dc.description.abstractIn this study, the structure of the purified extracellular eumelanin pigment isolated fromStreptomycesspp. was elucidated by detailed analysis via two different spectroscopic techniques (FT-IR and NMR). In vitro antiproliferative effects of eumelanin were evaluated on HeLa cell line. These experiments were carried out with the evaluation of the parameters including cell viability, cell index, and mitotic index. With the cell viability and cell index, IC50 concentration of eumelanin was determined as 10 mu M. This result showed that the IC50 concentration of eumelanin decreased the values of cell viability, cell index and mitotic index. These changes are statistically significant (p < 0.01). The ability of the dissolved eumelanin (250 mu g mL(-1)) to scavenge free radicals was determined via DPPH and ABTS and was shown to be about 87.73% and 75.2%, respectively, compared with standard antioxidants. It was observed that dry weights of eumelanin yield among the selected strains ranged from 160 to 240 mg L-1. The strain with the highest production potential was selected for 16S rDNA sequence analysis and, accordingly, the selected strain BSB49 was identified asStreptomyces parvusand the sequence analysis results were deposited in NCBI under accession number MK894155.en_US
dc.description.sponsorshipScientific Research Projects Coordination Unit of Bayburt University [2019/01-69001-04]en_US
dc.description.sponsorshipThis study was financially supported by Scientific Research Projects Coordination Unit of Bayburt University (project number: 2019/01-69001-04). We would like to thank Dr. Nesrin ECEM BAYRAM for her precious help in determining the antioxidant activity of eumelanin pigment.en_US
dc.identifier.doi10.1007/s00203-020-01956-2
dc.identifier.endpage2409en_US
dc.identifier.issn0302-8933
dc.identifier.issn1432-072X
dc.identifier.issue9en_US
dc.identifier.pmid32591909en_US
dc.identifier.scopus2-s2.0-85086841102en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2401en_US
dc.identifier.urihttps://doi.org/10.1007/s00203-020-01956-2
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3051
dc.identifier.volume202en_US
dc.identifier.wosWOS:000543620700002en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofArchives of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEumelaninen_US
dc.subjectBioproductionen_US
dc.subjectStructure elucidationen_US
dc.subjectAntiproliferative effecten_US
dc.subjectAntioxidant effecten_US
dc.titleBioproduction, structure elucidation and in vitro antiproliferative effect of eumelanin pigment fromStreptomyces parvusBSB49en_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu