Expression of cytosolic and noncytosolic carbonic anhydrase enzymes from bovine brain membrane

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dc.authorid35511537500
dc.authorid7006472528
dc.authorid7003959508
dc.authorid8064145700
dc.contributor.authorDemir N.
dc.contributor.authorDemir Y.
dc.contributor.authorCoskun F.
dc.contributor.authorTasgin E.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:44:38Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:44:38Z
dc.date.issued2012
dc.departmentBayburt Üniversitesien_US
dc.description.abstractCarbonic anhydrase is an enzyme that takes responsibility in inhalation function but, until today, carbonic anhydrase is not examined if it is present in the bovine brain membrane or not. The enzyme carbonic anhydrase was purified and separately characterized according to the bonding forms in 4 steps such as outer peripheral, cytosolic, inner peripheral and integral. Affinity chromatography was used for purification of the enzyme in all four steps. The affinity column was prepared with sepharose-4B-L-tyrosine-sulphanilamide. Purified carbonic anhydrase was obtained at each step. Enzyme activity was measured by CO 2 hydratase activity and esterase activity methods. Optimum pH and optimum temperature were defined for purified enzymes at each step. Morover molecular weight and purity were detected by gel filtration and SDS-PAGE electrophorose. In addition, the enzyme's Km and n max values were found with the Lineweaver- Burk method. The obtained results are discussed in comparison with other mammalian carbonic anhydrases. Carbonic anhydrase was shown to be exist in bovine brain membrane and this enzyme was optimized.en_US
dc.identifier.endpage1514
dc.identifier.issn0970-7077
dc.identifier.issue4
dc.identifier.scopus2-s2.0-84861668517en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage1511
dc.identifier.urihttps://hdl.handle.net/20.500.12403/900
dc.identifier.volume24
dc.identifier.wosWOS:000300464200023en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofAsian Journal of Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBrain membrane
dc.subjectCarbonic anhydrase
dc.subjectBrain membrane
dc.subjectCarbonic anhydrase
dc.titleExpression of cytosolic and noncytosolic carbonic anhydrase enzymes from bovine brain membraneen_US
dc.typeArticleen_US

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