Tailoring Physicochemical and Technological Properties of ?-Glucans Through Mutations of GTFA from Lactobacillus Reuteri E81

Basit Öğe Kaydı
dc.contributor.authorGokcan, Hande
dc.contributor.authorIspirli, Humeyra
dc.contributor.authorDertli, Enes
dc.date.accessioned2026-02-28T12:17:43Z
dc.date.available2026-02-28T12:17:43Z
dc.date.issued2026
dc.departmentBayburt Üniversitesi
dc.description.abstractGlucansucrases catalyze the production of alpha-glucans using sucrose as substrate with distinct physicochemical characteristics originates from mainly the type of linkages, degree of branching and molecular mass. New forms of alpha-glucans can be produced by applying specific mutations to glucansucrases. In this study, a single and double mutation at Leu933 and Leu935 sites of glucansucrase E81 was applied and L933I and L933I-L935F sites modified glucansucrase was obtained. Structural characterisation of bioengineered alpha-glucans revealed that ratio of (1 -> 3):(1 -> 6) linked alpha-Glc units altered at L933I whereas no (1 -> 3)-linked alpha-Glc units were presented for L933I-L935F modified glucansucrase detected by NMR analysis. Both bioengineered alpha-glucans had lower molecular weights in comparison to glucan E81 detected by GPC analysis. TGA and DSC analysis were applied to detect the thermal profiles of bioengineered alpha-glucans whereas FTIR and XRD analysis were applied to determine the alterations in the structural and physical nature of the bioengineered alpha-glucans, respectively. Finally, SEM and AFM analysis were applied to explore the morphological modifications of the bioengineered alpha-glucans. This study provides a better understanding for the potential role of final glucan structures on their physicochemical characteristics. Keywords: glucansucrase; carbohydrate engineering; mutation.
dc.description.sponsorshipTrkiye Bilimsel ve Teknolojik Arascedil;timath;rma Kurumu [121O007]
dc.description.sponsorshipThis study was supported by Scientific and Technological Research Council of Turkey (TUBITAK) under the Grant Number 121O007. The authors thank to TUBITAK for their supports.
dc.identifier.doi10.1007/s11483-026-10105-z
dc.identifier.issn1557-1858
dc.identifier.issn1557-1866
dc.identifier.issue1
dc.identifier.urihttps://doi.org/10.1007/s11483-026-10105-z
dc.identifier.urihttps://hdl.handle.net/20.500.12403/5942
dc.identifier.volume21
dc.identifier.wosWOS:001674423700002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofFood Biophysics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260218
dc.subjectGlucansucrase
dc.subjectCarbohydrate engineering
dc.subjectMutation
dc.titleTailoring Physicochemical and Technological Properties of ?-Glucans Through Mutations of GTFA from Lactobacillus Reuteri E81
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu