Interleukin-27 as a novel player in alleviating hepatic steatosis: Mechanistic insights from an in vitro analysis

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dc.authoridJung, Tae Woo/0000-0002-1167-1892
dc.authoridAbd El-Aty, A. M./0000-0001-6596-7907
dc.contributor.authorCho, Wonjun
dc.contributor.authorOh, Heeseung
dc.contributor.authorAbd El-Aty, A. M.
dc.contributor.authorOzten, Omer
dc.contributor.authorJeong, Ji Hoon
dc.contributor.authorJung, Tae Woo
dc.date.accessioned2024-10-04T18:51:08Z
dc.date.available2024-10-04T18:51:08Z
dc.date.issued2024
dc.departmentBayburt Üniversitesien_US
dc.description.abstractInterleukin-27 (IL -27) is a recently discovered cytokine that has been implicated in inflammatory and metabolic conditions, such as atherosclerosis and insulin resistance. However, the mechanisms by which IL -27 attenuates hepatic lipid accumulation in hyperlipidemic conditions and counteracts endoplasmic reticulum (ER) stress, a known risk factor for impaired hepatic lipid metabolism, have not been elucidated. This in vitro study was designed to examine the effect of IL -27 on hepatic lipid metabolism. The study included the evaluation of lipogenesis-associated proteins and ER stress markers by Western blotting, the determination of hepatic lipid accumulation by Oil Red O staining, and the examination of autophagosome formation by MDC staining. The results showed that IL -27 treatment reduced lipogenic lipid deposition and the expression of ER stress markers in cultured hepatocytes exposed to palmitate. Moreover, treatment with IL -27 suppressed CD36 expression and enhanced fatty acid oxidation in palmitate-treated hepatocytes. The effects of IL -27 on hyperlipidemic hepatocytes were attenuated when adenosine monophosphate-activated protein kinase (AMPK) or 3-methyladenine (3 MA) were inhibited by small interfering RNA (siRNA). These results suggest that IL -27 attenuates hepatic ER stress and fatty acid uptake and stimulates fatty acid oxidation via AMPK/autophagy signaling, thereby alleviating hepatic steatosis. In conclusion, this study identified IL -27 as a promising therapeutic target for nonalcoholic fatty liver disease (NAFLD).en_US
dc.description.sponsorshipNational Research Foundation of Korea (NRF) grant - Korea government (MSIT) [2022R1A2B5B01001453]; Chung-Ang Universityen_US
dc.description.sponsorshipThis work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2022R1A2B5B01001453) and by Chung-Ang University Research Grants in 2023.en_US
dc.identifier.doi10.1016/j.bbrc.2024.149671
dc.identifier.issn0006-291X
dc.identifier.issn1090-2104
dc.identifier.pmid38367515en_US
dc.identifier.scopus2-s2.0-85185529828en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.bbrc.2024.149671
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3402
dc.identifier.volume703en_US
dc.identifier.wosWOS:001188494300001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIL-27en_US
dc.subjectNAFLDen_US
dc.subjectER stressen_US
dc.subjectAMPKen_US
dc.subjectAutophagyen_US
dc.subjectObesityen_US
dc.titleInterleukin-27 as a novel player in alleviating hepatic steatosis: Mechanistic insights from an in vitro analysisen_US
dc.typeArticleen_US

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