Bifidogenic effect and in vitro immunomodulatory roles of melibiose-derived oligosaccharides produced by the acceptor reaction of glucansucrase E81

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dc.authoridKAYA, Yasemin/0000-0003-2760-8959
dc.authoridISPIRLI, HUMEYRA/0000-0002-7601-0374
dc.contributor.authorIspirli, Humeyra
dc.contributor.authorKaya, Yasemin
dc.contributor.authorDertli, Enes
dc.date.accessioned2024-10-04T18:48:24Z
dc.date.available2024-10-04T18:48:24Z
dc.date.issued2020
dc.departmentBayburt Üniversitesien_US
dc.description.abstractGlucansucrases are responsible for the production of alpha-glucans using sucrose as the substrate. Glucansucrases may also produce different oligosaccharides by transferring the glucose moiety from sucrose to a variety carbohydrates acting as acceptor nucleophile. In this study, melibiose-derived oligosaccharides were produced by the glucansucrase from Lactobacillus reuteri E81 expressed without the N-terminal region (GtfA-Delta N). The reaction products were characterized by TLC, LC-MS and NMR analysis and it was found that GtfA-Delta N synthesized melibiose-derived oligosaccharides (DP3 and DP4) by adding glucose units through alpha 1- > 3 or 1- > 6 glycosidic bond. The functional characteristics of these melibiose-derived oligosaccharides were determined by testing the immune-modulatory functions in HT-29 cells and testing their growth promoting effects for important probiotic and pathogenic strains. The melibiose-derived oligosaccharides triggered the production of anti-inflammatory cytokine IL-10 and pro-inflammatory cytokine TNF-alpha depending on their concentrations. Finally, melibiose-derived oligosaccharides showed bifidogenic effect as potential prebiotics.en_US
dc.description.sponsorshipTUBITAK (The Scientific and Technological research council of Turkey) [116O523]en_US
dc.description.sponsorshipThis study was financially supported by TUBITAK (The Scientific and Technological research council of Turkey) with the grant number 116O523.en_US
dc.identifier.doi10.1016/j.procbio.2019.12.003
dc.identifier.endpage131en_US
dc.identifier.issn1359-5113
dc.identifier.issn1873-3298
dc.identifier.scopus2-s2.0-85076551041en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage126en_US
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2019.12.003
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3042
dc.identifier.volume91en_US
dc.identifier.wosWOS:000526117900014en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofProcess Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlucansucraseen_US
dc.subjectMelibiose-Derived oligosaccharidesen_US
dc.subjectImmune-Modulationen_US
dc.subjectPrebioticsen_US
dc.subjectFunctional rolesen_US
dc.titleBifidogenic effect and in vitro immunomodulatory roles of melibiose-derived oligosaccharides produced by the acceptor reaction of glucansucrase E81en_US
dc.typeArticleen_US

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