Response surface methodology as optimization strategy for asymmetric bioreduction of acetophenone using whole cell of Lactobacillus senmaizukei

dc.authoridTaylan, Osman/0000-0002-5806-3237
dc.contributor.authorColak, Nida Sezin
dc.contributor.authorSahin, Engin
dc.contributor.authorDertli, Enes
dc.contributor.authorYilmaz, Mustafa Tahsin
dc.contributor.authorTaylan, Osman
dc.date.accessioned2024-10-04T18:52:49Z
dc.date.available2024-10-04T18:52:49Z
dc.date.issued2019
dc.departmentBayburt Üniversitesien_US
dc.description.abstractWhole cell applications are one of the main methodologies for the bioreduction of prochiral ketones to obtain enantiomerically rich chiral secondary alcohols which are mainly affected by the culture parameters of the whole cell. In this study, whole cell of Lactobacillus senmaizukei as a safe Lactic Acid Bacteria (LAB) was used for the reduction of acetophenone and Response Surface Methodology (RSM) application was used to optimize the culture parameters in terms of temperature, pH, incubation time, and agitation level to obtain the highest enantiomeric excess (ee) and conversion rate. The predicted optimum conditions for the bioreduction with whole cell Lactobacillus senmaizukei were found to be pH of 5.25, temperature of 25 degrees C, incubation time of 72 hr, and agitation level of 100 rpm. Importantly, the efficiency of the reduction of the acetophenone was significantly affected by the linear and quadratic effects of culture parameters. These findings are important to show the role of culture parameters for the bioreduction reactions and also the efficiency of the RSM technique to optimize these parameters.en_US
dc.identifier.doi10.1080/10826068.2019.1633668
dc.identifier.endpage890en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue9en_US
dc.identifier.pmid31259668en_US
dc.identifier.scopus2-s2.0-85068520979en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage884en_US
dc.identifier.urihttps://doi.org/10.1080/10826068.2019.1633668
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3661
dc.identifier.volume49en_US
dc.identifier.wosWOS:000473885700001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAsymmetric bioreductionen_US
dc.subjectbiotransformationen_US
dc.subjectRSMen_US
dc.subjectwhole-cell biocatalysten_US
dc.titleResponse surface methodology as optimization strategy for asymmetric bioreduction of acetophenone using whole cell of Lactobacillus senmaizukeien_US
dc.typeArticleen_US

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