Utilisation of an active branching sucrase from Lactobacillus kunkeei AP-37 to produce techno-functional poly-oligosaccharides

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dc.authoridKORKMAZ, Kader/0000-0002-8895-9210
dc.authoridTuncil, Yunus Emre/0000-0002-9421-2332
dc.contributor.authorIspirli, Huemeyra
dc.contributor.authorKorkmaz, Kader
dc.contributor.authorArioglu-Tuncil, Seda
dc.contributor.authorBozkurt, Fatih
dc.contributor.authorSagdic, Osman
dc.contributor.authorTuncil, Yunus Emre
dc.contributor.authorNarbad, Arjan
dc.date.accessioned2024-10-04T18:54:08Z
dc.date.available2024-10-04T18:54:08Z
dc.date.issued2023
dc.departmentBayburt Üniversitesien_US
dc.description.abstractGlucansucrase AP-37 was extracted from the culture supernatant of Lactobacillus kunkeei AP-37 and character-istics of the glucan produced by the active glucansucrase in terms of structural and functional roles were determined in this study. A molecular weight around 300 kDa was observed for glucansucrase AP-37 and its acceptor reactions with maltose, melibiose and mannose were also conducted to unveil the prebiotic potential of the poly-oligosaccharides formed via these reactions. The core structure of glucan AP-37 was determined by 1H and 13C NMR and GC/MS analysis which revealed that glucan AP-37 was a highly branched dextran composing of high levels of (1-+ 3)-linked alpha-D-glucose units with low levels of (1-+ 2)-linked alpha-D-glucose units. The structural features of the glucan formed, demonstrated that glucansucrase AP-37 was an alpha-(1-+ 3) branching sucrase. Dextran AP-37 was further characterised by FTIR analysis and XRD analysis demonstrated its amorphous nature. A fibrous compact morphology was observed for dextran AP-37 with SEM analysis whereas TGA and DSC analysis revealed its high stability as no degradation was observed up to 312 degrees C. Finally, the prebiotic potential of the dextran AP-37 and the gluco-oligosaccharides produced with the acceptor reaction of alpha-(1-+ 3) branching sucrase AP-37 were determined and promising results were found for the gluco-oligosaccharides to act as prebiotics.en_US
dc.description.sponsorshipYildiz Technical University Scientific Research Projects (BAP); Turkish Academy of Sciences (TUEBA); [FBA-2021-4206]en_US
dc.description.sponsorshipThis study is funded by Yildiz Technical University Scientific Research Projects (BAP) with the project number FBA-2021-4206. The partial support of Turkish Academy of Sciences (TUEBA) to this study is also greatly acknowledged.en_US
dc.identifier.doi10.1016/j.ijbiomac.2023.123967
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid36906201en_US
dc.identifier.scopus2-s2.0-85149862010en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2023.123967
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3869
dc.identifier.volume236en_US
dc.identifier.wosWOS:000955699000001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBranching sucraseen_US
dc.subjectBranched dextranen_US
dc.titleUtilisation of an active branching sucrase from Lactobacillus kunkeei AP-37 to produce techno-functional poly-oligosaccharidesen_US
dc.typeArticleen_US

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