Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40

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dc.authorid0009-0001-5794-4497
dc.authorid0000-0002-8895-9210
dc.authorid0000-0002-9421-2332
dc.contributor.authorDere, Sevda
dc.contributor.authorIspirli, Humeyra
dc.contributor.authorKorkmaz, Kader
dc.contributor.authorTuncil, Yunus Emre
dc.contributor.authorSagdic, Osman
dc.contributor.authorNarbad, Arjan
dc.contributor.authorDertli, Enes
dc.date.accessioned2026-02-28T12:17:55Z
dc.date.available2026-02-28T12:17:55Z
dc.date.issued2025
dc.departmentBayburt Üniversitesi
dc.description.abstractGlucansucrases (GS) are functional enzymes for the production of distinct glucan and gluco-oligosaccharide structures with potential bioactive characteristics. In this study, an active glucansucrase (GS40) was identified within the genome of Leuconostoc mesenteroides YTU 40 that was further cloned, heterologously expressed and biochemically characterised. GS40 was capable of production of a branched dextran with similar to 20% (1 -> 3)-alpha-D-Glc residues detected by(1)H and(13)C NMR analysis. Acceptor reactions of GS40 with maltose and cellobiose were performed and distinct maltose/cellobiose derivative acetylated gluco-oligosaccharides were produced detected by TLC, NMR and LC-MS analysis. The bioactive characteristics of branched dextran and gluco-oligosaccharides were tested by determination of their immune-modulatory roles in terms of triggering IL-12, IL-10, IL-4 and TNF-alpha cytokine levels. These findings can be important to unveil the role of glucansucrases for the production of gluco-oligosaccharides that can be used as functional ingredients for food and other industries.
dc.description.sponsorshipYildiz Teknik niversitesi [FBI-2022-5143]; Yimath;ldimath;z Technical University Scientific Research Projects (BAP) [12F049]; United Arab Emirates University (UAEU)
dc.description.sponsorshipThis study was funded by Y & imath;ld & imath;z Technical University Scientific Research Projects (BAP) with the project number FBI-2022-5143 and United Arab Emirates University (UAEU) via project 12F049. The authors also thank James Choi for technical assistance during the whole genome sequencing. This study is part of Sevda Dere's Master's Thesis.
dc.identifier.doi10.1038/s41598-025-96324-9
dc.identifier.issn2045-2322
dc.identifier.issue1
dc.identifier.pmid40181138
dc.identifier.scopus2-s2.0-105002852685
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1038/s41598-025-96324-9
dc.identifier.urihttps://hdl.handle.net/20.500.12403/6025
dc.identifier.volume15
dc.identifier.wosWOS:001459337000027
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherNature Portfolio
dc.relation.ispartofScientific Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260218
dc.subjectBIOCHEMICAL-CHARACTERIZATION
dc.subjectACCEPTOR REACTIONS
dc.subjectALPHA
dc.subjectSUCROSE
dc.subjectFRUCTOOLIGOSACCHARIDES
dc.subjectPOLYSACCHARIDES
dc.titleCharacterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40
dc.typeArticle

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