Impact of gossypin on gene expression of HSP60 and HSP70 in different cancer cell lines

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dc.authoridCINAR, IRFAN/0000-0002-9826-2556
dc.contributor.authorDirican, Ebubekir
dc.contributor.authorCinar, Trfan
dc.date.accessioned2024-10-04T18:51:06Z
dc.date.available2024-10-04T18:51:06Z
dc.date.issued2022
dc.departmentBayburt Üniversitesien_US
dc.description.abstractPurpose: The aim of this study is to evaluate the impact of gossypin on the expression level of heat shock proteins (HSPs) genes in different cancer cells. Materials and Methods: Cells were grown under standard culture conditions. Cancer cells were treated with different concentrations (5-100 mu g/ml) of gossypin and cisplatin (50 mu M) as positive control. Cell viability and effective dose range (5-100 mu g/ml) of gossypin were determined by MTT at 24, 48 and 72 hours. After RNA isolation and cDNA synthesis, HSP60 and HSP70 gene expression levels were analyzed using RT-PCR. For gene expression analysis, the 2-(Delta Delta ct) method was used. Results: According to the Mil results, 25-50-100 mu g/ml of gossypin doses were found effective on HSP60 and HSP70 gene expression levels in the cancer cell lines. Gossypin affected with dose-dependently the expression of HSP60 and HSP70 in the three cell lines. In the three cell lines, 50 mu g/ml and 100 mu g/ml of gossypin doses significantly reduced the expression of HSP60 and HSP70 compared to control group. Conclusion: Our results strongly supported the anticarcinogenic effect of gossypin at various doses in different cell lines. However, we believe that further in vivo research and human studies are needed. Our findings suggest that gossypin could be suitable candidate agent for further investigation to develop new strategies for the prevention and/or treatment of different cancer types.en_US
dc.identifier.doi10.17826/cumj.1052787
dc.identifier.endpage503en_US
dc.identifier.issn2602-3032
dc.identifier.issn2602-3040
dc.identifier.issue2en_US
dc.identifier.startpage497en_US
dc.identifier.trdizinid524575en_US
dc.identifier.urihttps://doi.org/10.17826/cumj.1052787
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/524575
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3382
dc.identifier.volume47en_US
dc.identifier.wosWOS:000815481700003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherCukurova Univ, Fac Medicineen_US
dc.relation.ispartofCukurova Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGossypinen_US
dc.subjectHSP60en_US
dc.subjectHSP70en_US
dc.subjectMCF7en_US
dc.subjectPC3en_US
dc.subjectHep3ben_US
dc.titleImpact of gossypin on gene expression of HSP60 and HSP70 in different cancer cell linesen_US
dc.typeArticleen_US

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