Investigation of the relationship between NFKB1 polymorphisms and telomere length and apoptosis in patients with type-2 diabetes

Basit Öğe Kaydı
dc.authoridkaya, yasemin/0000-0001-7360-8090
dc.authoridDirican, Ebubekir/0000-0001-9260-5223
dc.contributor.authorDirican, Ebubekir
dc.contributor.authorKaya, Yasemin
dc.date.accessioned2024-10-04T18:51:16Z
dc.date.available2024-10-04T18:51:16Z
dc.date.issued2023
dc.departmentBayburt Üniversitesien_US
dc.description.abstractPurpose: Type 2 diabetes mellitus (T2DM) is a heterogeneous, chronic, and metabolic disease that affects a significant proportion of the global population. This study aimed to evaluate the effect of NFKB1-94 ATTG ins/del polymorphisms on the expression of apoptosis genes and telomere length (TL) in patients with T2DM compared with healthy individuals.Materials and Methods: Sixty-nine T2DM patients and sixty healthy people were enrolled in the study. DNA and RNA were isolated from the blood samples. NFKB1 genotypes were identified by Sanger sequencing. For TL analyses and to investigate the expression of the caspase-3, caspase-9, bax, and bcl2 genes, RT-PCR was utilized.Results: There was a significant difference between the NFKB1-94 ins/del genotype patients and the control group (OR:0.4792 (0.2345-1.011)). However, the distribution of other genotype/alleles (ins/ins and del/del) showed no difference between T2DM and control groups. The allelic frequency of NFKB1-94 ins/del was 0.455/0.235 for the T2DM group and 0.435/0.165 for the control group. An increase in the mRNA expression of caspase-3, caspase-9 and Bax genes was observed in the T2DM group compared with the healthy group, while a decrease in the Bcl2 gene was found in the T2DM group. TL in T2DM patients was shorter than in healthy individuals.Conclusion: NFKB1-94 ins/del polymorphisms show significant differences in T2DM patients. We observed that apoptosis was activated and TL was shortened in patients with T2DM. However, no relationship between NFKB1 polymorphisms and apoptosis and TL could not be determined.en_US
dc.description.sponsorshipBayburt University Scientific Research Project Coordinator (BAU-BAP) [2021/69001-01-10]en_US
dc.description.sponsorshipThis study was supported by Bayburt University Scientific Research Project Coordinator (BAU-BAP-Number: 2021/69001-01-10).en_US
dc.identifier.doi10.17826/cumj.1238482
dc.identifier.endpage226en_US
dc.identifier.issn2602-3032
dc.identifier.issn2602-3040
dc.identifier.issue1en_US
dc.identifier.startpage216en_US
dc.identifier.trdizinid1181518en_US
dc.identifier.urihttps://doi.org/10.17826/cumj.1238482
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1181518
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3447
dc.identifier.volume48en_US
dc.identifier.wosWOS:000960019300026en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherCukurova Univ, Fac Medicineen_US
dc.relation.ispartofCukurova Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectT2DMen_US
dc.subjectNFKB1en_US
dc.subjectapoptosisen_US
dc.subjecttelomere lengthen_US
dc.subjectSanger sequencingen_US
dc.titleInvestigation of the relationship between NFKB1 polymorphisms and telomere length and apoptosis in patients with type-2 diabetesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu