Production of mannose-containing oligosaccharides by glucansucrase E81 and determination of their functional characteristics

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dc.authoridISPIRLI, HUMEYRA/0000-0002-7601-0374
dc.contributor.authorIspirli, Humeyra
dc.contributor.authorDertli, Enes
dc.date.accessioned2024-10-04T18:52:44Z
dc.date.available2024-10-04T18:52:44Z
dc.date.issued2020
dc.departmentBayburt Üniversitesien_US
dc.description.abstractOligosaccharides are one of the functional ingredients to be used in food technology. In this study, by using an active glucansucrase GTFA-Delta N E81 in the acceptor reaction of mannose, mannose-containing oligosaccharides were produced and their functionality was tested. The formation of the oligosaccharides were visualised by TLC analysis and mannose-containing oligosaccharides up to DP 7 were determined by LC-MS analysis. The presence of the (1,6)Glc and (1,3)Glc units within the oligosaccharides were determined by NMR analysis. The in vitro immune-modulatory functions of the mannose-containing oligosaccharides were determined but no induction in IL-4, IL-10, IL-12 and TNF-alpha cytokine levels were detected. Importantly this oligosaccharide mixture showed prebiotic effect by triggering the growth of tested probiotics and not affecting the growth of pathogen strains. Our findings reveals the potential of the role of glucansucrases for the production of functional oligosaccharides.en_US
dc.description.sponsorshipTUBITAK [116O523]en_US
dc.description.sponsorshipThis study was financially supported by TUBITAK with the grant number [116O523].en_US
dc.identifier.doi10.1080/10242422.2019.1661384
dc.identifier.endpage209en_US
dc.identifier.issn1024-2422
dc.identifier.issn1029-2446
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85072023029en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage202en_US
dc.identifier.urihttps://doi.org/10.1080/10242422.2019.1661384
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3633
dc.identifier.volume38en_US
dc.identifier.wosWOS:000485975200001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofBiocatalysis and Biotransformationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlucansucraseen_US
dc.subjectmannose-containing oligosaccharidesen_US
dc.subjectimmune modulationen_US
dc.subjectprebioticen_US
dc.titleProduction of mannose-containing oligosaccharides by glucansucrase E81 and determination of their functional characteristicsen_US
dc.typeArticleen_US

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