Synthesis and characterization of Bifidogenic raffinose-derived oligosaccharides via acceptor reactions of glucansucrase E81

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dc.authoridSkory, Christopher/0000-0001-6268-9104
dc.contributor.authorIspirli, Humeyra
dc.contributor.authorBowman, Michael J.
dc.contributor.authorSkory, Christopher D.
dc.contributor.authorDertli, Enes
dc.date.accessioned2024-10-04T18:53:49Z
dc.date.available2024-10-04T18:53:49Z
dc.date.issued2021
dc.departmentBayburt Üniversitesien_US
dc.description.abstractGlucansucrases can perform acceptor reactions with different sugars to produce distinct oligosaccharides which can potentially act as prebiotics depending on their characteristics. Using cheap substrate sucrose and under mild and environmentally friendly conditions, glucansucrase-derived oligosaccharides can be produced with high yields. In this study, raffinose-derived oligosaccharides up to degree of polymerization (DP) 7 produced by glucansucrase E81 were characterized and their prebiotic functions were evaluated. The main product was a DP 4 oligosaccharide containing two products formed by the transfer of the glucose to raffinose at either the 3- (81%) or 4-O (19%) position of its galactose residue confirmed by NMR spectroscopy and LC-MS-LTQ analysis. The purified DP 4 raffinose-derived oligosaccharides were subjected to in vitro human gastric fluid and a-amylase digestion tests and no digestion was observed under both cases. Finally, prebiotic potential of DP 4 raffinose-derived oligosaccharides was tested with probiotic and pathogenic strains and only growth of Bifidobacteria promoted, thus supported the Bifidogenic activity of DP 4 raffinose-derived oligosaccharides.en_US
dc.description.sponsorshipTUBITAK [116O523]; U.S. Department of Agriculture, Agricultural Research Serviceen_US
dc.description.sponsorshipThe authors are thankful to TUBITAK for the financial support for this study with the grant number 116O523. This work was supported in part by the U.S. Department of Agriculture, Agricultural Research Service.en_US
dc.identifier.doi10.1016/j.lwt.2021.111525
dc.identifier.issn0023-6438
dc.identifier.issn1096-1127
dc.identifier.scopus2-s2.0-85104722448en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.lwt.2021.111525
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3718
dc.identifier.volume147en_US
dc.identifier.wosWOS:000685057500005en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofLwt-Food Science and Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectStructural-Characterizationen_US
dc.subjectGalacto-Oligosaccharidesen_US
dc.subjectAlternansucraseen_US
dc.subjectPrebioticsen_US
dc.titleSynthesis and characterization of Bifidogenic raffinose-derived oligosaccharides via acceptor reactions of glucansucrase E81en_US
dc.typeArticleen_US

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