Production of lactose derivative hetero-oligosaccharides from whey by glucansucrase E81 and determination of prebiotic functions

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dc.contributor.authorIspirli, Humeyra
dc.contributor.authorDertli, Enes
dc.date.accessioned2024-10-04T18:52:44Z
dc.date.available2024-10-04T18:52:44Z
dc.date.issued2021
dc.departmentBayburt Üniversitesien_US
dc.description.abstractBioactive oligosaccharides are important compounds for development of functional foods and ingredients. In this study, lactose derivative hetero-oligosaccharides were produced by acceptor reaction of glucansucrase E81 with whey. The synthesis of oligosaccharides was monitored by Thin Layer Chromatography (TLC) analysis followed by (1 ) and C-13 Nuclear Magnetic Resonance (NMR) spectroscopy analysis which demonstrated the glycosylation of lactose with alpha-(1 -> 2), alpha-(1 -> 3) and alpha-(1 -> 4) linkages. Liquid chromatography-mass spectrometry (LC-MS) analysis demonstrated the formation of DP 7 oligosaccharides from whey. The synthesized oligosaccharides were not digested under in vitro human gastric juice and alpha-amylase conditions. The prebiotic effect of lactose derivative hetem-oligosaccharides was tested and no growth in pathogenic strains was observed whereas certain probiotic strains utilised lactose derivative hetem-oligosaccharides as Carbon source and their growth was promoted. These findings can be important to reveal the potential of glucansucrases to produce functional oligosaccharides from different sources including waste materials.en_US
dc.description.sponsorshipTUBITAK [116O523]en_US
dc.description.sponsorshipThe authors are thankful to TUBITAK for the financial support for this study with the grant number 116O523.en_US
dc.identifier.doi10.1016/j.lwt.2020.110471
dc.identifier.issn0023-6438
dc.identifier.issn1096-1127
dc.identifier.scopus2-s2.0-85094583823en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.lwt.2020.110471
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3632
dc.identifier.volume137en_US
dc.identifier.wosWOS:000600557500096en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofLwt-Food Science and Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlucansucraseen_US
dc.subjectOligosaccharidesen_US
dc.subjectWheyen_US
dc.subjectPrebioticen_US
dc.titleProduction of lactose derivative hetero-oligosaccharides from whey by glucansucrase E81 and determination of prebiotic functionsen_US
dc.typeArticleen_US

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