RP-HPLC-UV Analysis of the Phenolic Compounds, Antimicrobial Activity Against Multi-Drug Resistant Bacteria and Antioxidant Activity of Fruit and Seed of Diospyros lotus L.

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dc.contributor.authorAkar, Zeynep
dc.contributor.authorKarakurt, Aylin
dc.contributor.authorOkumus, Funda
dc.contributor.authorCinemre, Sedanur
dc.contributor.authorDuzgun, Azer Ozad
dc.contributor.authorAkar, Bulent
dc.contributor.authorCan, Zehra
dc.date.accessioned2024-10-04T18:58:48Z
dc.date.available2024-10-04T18:58:48Z
dc.date.issued2020
dc.departmentBayburt Üniversitesien_US
dc.description.abstractThe object of the present study was to investigate the antioxidant, antimicrobial activity and phenolic compounds of fruit and seeds of Diospyros lotus L. The fruits consumed by humans as nutrients were harvested from the plants that grow naturally in the province of Trabzon-Akçaabat, Eastern Black Sea Region of Turkey. The antioxidant activities of fruit and seeds of Diospyros lotus were determined by using four methods (% DPPH radical scavenging activity, FRAP antioxidant power determination, CUPRAC reducing antioxidant activity and total phenolic content (TPC) in five different proportion of methanol-water extracts. All extracts of fruit and seeds of D. lotus analyzed through whole antioxidant analysis methods showed significant antioxidant activity. In addition, antimicrobial activity of fruit and seeds extracted with DMSO was determined against seven standard bacteria and three multi-drug resistance clinical strains. Although fruit extracts did not have the antimicrobial activity against bacteria, seeds showed antimicrobial effect to both standard strains (Gram-negative and Gram-positive) and antibiotic resistance clinical isolates (Klebsiella pneumoniae carrying blaKPC, Acinetobacter baumannii, harboring blaOXA-23 gene and resistance to colistin, and multi-drug resistant Pseudomonas aeruginosa). MICs’ value of plant seed extracts for standard strains was 0.75-25 mg/mL and antibiotic resistant clinical bacteria were 12.5 and 25 mg/mL. Additionally, phenolic compounds in methanol extracts of fruit and seeds were also determined in by HPLC using 19 standards. Gallic acid and chrysin phenolic compounds were the major phenolic compounds in fruit and seeds, respectively. © IJSM 2020.en_US
dc.identifier.doi10.21448/ijsm.714108
dc.identifier.endpage246en_US
dc.identifier.issn2148-6905
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85111568068en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage237en_US
dc.identifier.trdizinid466217en_US
dc.identifier.urihttps://doi.org/10.21448/ijsm.714108
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/466217
dc.identifier.urihttp://hdl.handle.net/20.500.12403/4037
dc.identifier.volume7en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherPamukkale Universityen_US
dc.relation.ispartofInternational Journal of Secondary Metaboliteen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntioxidanten_US
dc.subjectDiospyros lotusen_US
dc.subjectMulti-drug Resistant Bacteriaen_US
dc.subjectPhenolic Compoundsen_US
dc.titleRP-HPLC-UV Analysis of the Phenolic Compounds, Antimicrobial Activity Against Multi-Drug Resistant Bacteria and Antioxidant Activity of Fruit and Seed of Diospyros lotus L.en_US
dc.typeArticleen_US

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