Investigation of the effect of gossypin on MMP-2 and MMP- 9 genes in prostate cancer cells

Basit Öğe Kaydı
dc.authoridCINAR, IRFAN/0000-0002-9826-2556
dc.contributor.authorDirican, Ebubekir
dc.contributor.authorCinar, Irfan
dc.date.accessioned2024-10-04T18:51:14Z
dc.date.available2024-10-04T18:51:14Z
dc.date.issued2022
dc.departmentBayburt Üniversitesien_US
dc.description.abstractPurpose: The aim of this study is to explore the effects of gossypin on matrix metalloproteinases -2 (MMP-2) and MMP-9 genes in prostate cancer cells. Materials and Methods: PC3 cells were grown in culture and treated with three different concentrations of gossypin (25-50-100 mu g/ml) and cisplatin (50 mu M) as a positive control. First, RNA isolation was performed. Then, cDNA synthesis was performed and RT-PCR was used to evaluate mRNA expression levels of MMP-2 and MMP-9 genes. Results: Gossypin decreased MMP-2 and MMP-9 mRNA expression in prostate cancer cells in a concentrationdependent manner. Three concentrations (25-50-100 mu g/ml) of gossypin in PC3 cells reduced the mRNA expression of the MMP-2 gene. While the fold change value of MMP-2 gene expression was 0.3482 +/- 0.040 in the 100 mu g/ml gossypin group, it was 1.007 +/- 0.1425 in the control group. In addition, 50 mu g/ml and 100 mu g/ml concentrations of gossypin decreased the mRNA expression of the MMP-9 gene. The expression level of the MMP-9 gene in prostate cancer cells was 0.4740 +/- 0.038 in the 100 mu g/ml gossypin administered group, while it was 1.009 +/- 0.1687 in the control group. There was a positive correlation between the expressions of the MMP-2 and MMP-9 genes. Conclusion: According to the results obtained, it is seen that gossypin reduces the expression of MMP-2 and MMP9 genes in prostate cancer cells and the effects of gossypin on other genetic and epigenetic mechanisms in cancer need to be investigated to reveal the anti-cancer.en_US
dc.identifier.doi10.17826/cumj.1137306
dc.identifier.endpage1295en_US
dc.identifier.issn2602-3032
dc.identifier.issn2602-3040
dc.identifier.issue3en_US
dc.identifier.startpage1290en_US
dc.identifier.trdizinid1122232en_US
dc.identifier.urihttps://doi.org/10.17826/cumj.1137306
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1122232
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3437
dc.identifier.volume47en_US
dc.identifier.wosWOS:000889635700042en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherCukurova Univ, Fac Medicineen_US
dc.relation.ispartofCukurova Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGossypinen_US
dc.subjectMMP-2en_US
dc.subjectMMP-9en_US
dc.subjectPC-3en_US
dc.subjectRT-PCRen_US
dc.titleInvestigation of the effect of gossypin on MMP-2 and MMP- 9 genes in prostate cancer cellsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu