Characterizations and In Vitro Gut Microbiome Modulatory Effects of Gluco-Oligosaccharides Synthesized by the Acceptor Reactions of Glucansucrase 53

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dc.authorid0009-0001-5794-4497
dc.authorid0000-0002-5723-2959
dc.authorid0000-0002-9421-2332
dc.authorid0000-0002-8790-9529
dc.authorid0000-0002-5132-9733
dc.authorid0000-0002-2063-1462
dc.contributor.authorBayaman, Rabia Yusra
dc.contributor.authorAlkay, Zuhal
dc.contributor.authorIspirli, Humeyra
dc.contributor.authorArioglu-Tuncil, Seda
dc.contributor.authorDere, Sevda
dc.contributor.authorCan, Hasan
dc.contributor.authorDertli, Enes
dc.date.accessioned2026-02-28T12:18:15Z
dc.date.available2026-02-28T12:18:15Z
dc.date.issued2025
dc.departmentBayburt Üniversitesi
dc.description.abstractThe production of novel oligosaccharides with potential prebiotic effects is of interest to expand the current market and explore the effectiveness of new functional carbohydrate forms. The utilization of glucansucrases is a cost-effective and environmentally friendly biotechnological strategy for producing novel gluco-oligosaccharides through acceptor reactions. In this study, an active glucansucrase (GS53) was used to produce gluco-oligosaccharides via its acceptor reactions with glucose, maltose, and maltotriose, and these oligosaccharides were tested in terms of structure and their gut microbiome modulatory effects. The formations of oligosaccharides were monitored by TLC analysis, and GS53 was active for the three acceptors but not for the other sugars tested. The structural characterization of the gluco-oligosaccharides by 1H NMR analysis revealed the glycosylation of each acceptor with alpha-(1 -> 3) and alpha-(1 -> 6) linkages, whereas LC-MS analysis demonstrated the formations of DP 8, DP 7, and DP 6 oligosaccharides with acceptors maltose, maltotriose, and glucose, respectively. In vitro fecal fermentation analysis, in which microbial short-chain fatty acids (SCFAs) and microbial compositional changes were assessed using gas chromatography and 16S rRNA sequencing, respectively, demonstrated that the gluco-oligosaccharides formed SCFAs-particularly propionate and butyrate-at levels comparable to those observed with inulin, a well-established prebiotic. Additionally, the gluco-oligosaccharides were found to promote the growth of Bifidobacterium adolescentis and Blautia OTUs, which are known to have important physiological functions beneficial to human health. Overall, these results demonstrate that gluco-oligosaccharides synthesized using GS53 through acceptor reactions exhibit prebiotic potentials and could be utilized in the future as dietary supplements as well as in the development of functional foods targeting colonic health.
dc.description.sponsorshipTurkish Academy of Sciences via the TUEBA-GEBIdot;P award
dc.description.sponsorshipThis study was funded by Turkish Academy of Sciences via the TUEBA-GEB & Idot;P award given to Enes Dertli.
dc.identifier.doi10.3390/fermentation11060324
dc.identifier.issn2311-5637
dc.identifier.issue6
dc.identifier.scopus2-s2.0-105008902068
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/fermentation11060324
dc.identifier.urihttps://hdl.handle.net/20.500.12403/6189
dc.identifier.volume11
dc.identifier.wosWOS:001515554800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofFermentation-Basel
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260218
dc.subjectglucansucrases
dc.subjectacceptor reactions
dc.subjectgluco-oligosaccharides
dc.subjectshort chain fatty acids
dc.subjectbutyrate
dc.subjectpropionate
dc.subjectgut microbiome
dc.titleCharacterizations and In Vitro Gut Microbiome Modulatory Effects of Gluco-Oligosaccharides Synthesized by the Acceptor Reactions of Glucansucrase 53
dc.typeArticle

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