Purification and biochemical characterization of a novel copper, zinc superoxide dismutase from liver of camel (Camelus dromedarius): An antioxidant enzyme with unique properties

Basit Öğe Kaydı
dc.authorid57190409749
dc.authorid6507478596
dc.authorid56400331100
dc.authorid7004132795
dc.contributor.authorChafik A.
dc.contributor.authorEssamadi A.
dc.contributor.authorÇelik S.Y.
dc.contributor.authorMavi A.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:42:57Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:42:57Z
dc.date.issued2019
dc.departmentBayburt Üniversitesien_US
dc.description.abstractA novel copper, zinc superoxide dismutase (CuZnSOD) was purified to homogeneity from the liver of an animal well adapted to the stressful living conditions of the desert, the camel (Camelus dromedarius). The biochemical properties of camel liver CuZnSOD were examined. The purified enzyme had a native molecular weight of 28 kDa, as judged by gel filtration chromatography, and showed a single band at 27 kDa on SDS-PAGE, indicating that it is a monomeric protein. Optimal activity of the purified enzyme occurred at 43 °C and pH 6.0, and the activation energy was 1.42 kJ/mol. CuZnSOD activity was strongly inhibited by ?-ME, DTT, H 2 O 2 and SDS and slightly inhibited by EDTA, NaN 3 and PMSF. Al 3+ , Ca 2+ , Cd 2+ , Mg 2+ and Zn 2+ stimulated CuZnSOD activity, whereas Ba 2+ , Co 2+ , Fe 2+ and Ni 2+ inhibited it. The purified enzyme contained 0.010 µg of Cu and 0.69 µg of Zn per mg of protein. K m , V max , k cat and k cat /K m values for NBT and riboflavin were 16.27 and 0.16 µM, 20.85 and 21.54 U/mg, 9.65 and 9.97 s ?1 , and 0.59 and 62.33 s -1 µM ?1 , respectively. Camel liver CuZnSOD exhibited unique biochemical properties compared to those of other CuZnSODs, including lower molecular weight with a monomeric structure, higher optimum temperature, very low E a , very low optimum pH, very low contents of Cu and Zn, and higher affinity, turnover number and catalytic efficiency for riboflavin. These unique properties of camel liver CuZnSOD might be related to the ability of this animal to inhabit stressful desert conditions. © 2019 Elsevier Inc.en_US
dc.identifier.doi10.1016/j.bioorg.2019.02.024
dc.identifier.endpage436
dc.identifier.issn0045-2068
dc.identifier.pmid30771689en_US
dc.identifier.scopus2-s2.0-85061405625en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage428
dc.identifier.urihttps://dx.doi.org/10.1016/j.bioorg.2019.02.024
dc.identifier.urihttps://hdl.handle.net/20.500.12403/302
dc.identifier.volume86
dc.identifier.wosWOS:000464108100045en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc.
dc.relation.ispartofBioorganic Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiochemical characterization
dc.subjectCamel
dc.subjectCamelus dromedarius
dc.subjectCopper, zinc superoxide dismutase
dc.subjectPurification
dc.subjectStressful desert conditions
dc.subjectUnique properties
dc.subjectcopper zinc superoxide dismutase
dc.subjectcuprous ion
dc.subjectferrous ion
dc.subjectmagnesium ion
dc.subjectriboflavin
dc.subjectsodium azide
dc.subjectzinc ion
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectbiochemical analysis
dc.subjectcatalytic efficiency
dc.subjectcontrolled study
dc.subjectdesert
dc.subjectdromedary
dc.subjectenvironmental stress
dc.subjectenzyme activity
dc.subjectenzyme purification
dc.subjectgel filtration chromatography
dc.subjectkinetic parameters
dc.subjectliver homogenate
dc.subjectmolecular weight
dc.subjectnonhuman
dc.subjectoxidative stress
dc.subjectpH
dc.subjectpolyacrylamide gel electrophoresis
dc.subjectpriority journal
dc.subjectprotein determination
dc.subjectturnover number
dc.subjectzymography
dc.subjectBiochemical characterization
dc.subjectCamel
dc.subjectCamelus dromedarius
dc.subjectCopper, zinc superoxide dismutase
dc.subjectPurification
dc.subjectStressful desert conditions
dc.subjectUnique properties
dc.subjectcopper zinc superoxide dismutase
dc.subjectcuprous ion
dc.subjectferrous ion
dc.subjectmagnesium ion
dc.subjectriboflavin
dc.subjectsodium azide
dc.subjectzinc ion
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectbiochemical analysis
dc.subjectcatalytic efficiency
dc.subjectcontrolled study
dc.subjectdesert
dc.subjectdromedary
dc.subjectenvironmental stress
dc.subjectenzyme activity
dc.subjectenzyme purification
dc.subjectgel filtration chromatography
dc.subjectkinetic parameters
dc.subjectliver homogenate
dc.subjectmolecular weight
dc.subjectnonhuman
dc.subjectoxidative stress
dc.subjectpH
dc.subjectpolyacrylamide gel electrophoresis
dc.subjectpriority journal
dc.subjectprotein determination
dc.subjectturnover number
dc.subjectzymography
dc.titlePurification and biochemical characterization of a novel copper, zinc superoxide dismutase from liver of camel (Camelus dromedarius): An antioxidant enzyme with unique propertiesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu