Effect of different carbon sources on exopolysaccharide production of Lactiplantibacillus pentosus KS27 and optimization of production conditions using Box-Behnken designing
Küçük Resim Yok
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Heidelberg
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study aimed to determine the exopolysaccharide (EPS) production of Lactiplantibacillus pentosus KS27 depending on temperature, incubation time, and medium composition and to determine the optimum conditions. Characterization was carried out for the exopolysaccharide produced under optimum conditions. In the study, three different conditions (25 degrees C, 35 degrees C, 45 degrees C) were used for temperature, glycerol, and sucrose as media, and three different periods (24 h, 36 h, and 48 h) were used. It was determined that the solubility of EPS produced under optimal conditions had the highest water content (98 +/- 0.01%), while the water binding capacity was also found to be as high as 1970 +/- 0.01% and complied with common EPS characteristics. Differential scanning calorimeter analysis showed a gradual deterioration up to 191.88 degrees C and a slow decline up to 400 degrees C. FTIR analysis deciphered peak values between 3289 and 517 cm(-1), and functional groups were detected. When the nuclear magnetic resonance (NMR) spectrum was examined, a heterogeneous structure appropriate to the structure of EPS was found by defining the peak entities in three separate regions.
Açıklama
Anahtar Kelimeler
Exopolysaccharide, Optimization, Sucrose, Glycerol, Lactiplantibacillus pentosus
Kaynak
Biomass Conversion and Biorefinery
WoS Q Değeri
Q2
Scopus Q Değeri
Q3
