Characterisation of dextran AP-27 produced by bee pollen isolate Lactobacillus kunkeei AP-27
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Sci Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An exopolysaccharide (EPS) produced by bee pollen isolate Lactobacillus kunkeei AP-27 was characterised in this study. EPS monomeric composition of strain AP-27 was characterised by High-performance liquid chromatography (HPLC) analysis and glucose was the only sugar in the EPS repeating unit. Structural analysis of glucan AP27 by H-1 and C-13 Nuclear magnetic resonance (NMR) analysis demonstrated that this glucan was a dextran ((1-*6)-linked alpha-D-glucose units as the main body) consisting very low level of (1-* 4)-linked alpha-D-glucose units (similar to 0.5 %). Dextran AP-27 had a degradation temperature of 308 degrees C that revealed its superior thermal strength identified by Thermogravimetric (TGA) and Differential scanning calorimetry (DSC) analysis. The molecular weight of dextran AP-27 was determined to be 25 kDa by Gel Permeation Chromatography (GPC) analysis. Crystallographic properties of dextran AP-27 was determined by X-ray diffraction (XRD) analysis that divulged its amorphous feature. Scanning Electron Microscopy (SEM) analysis was applied for microstructural characterisation of dextran AP-27 which revealed its regular sphere like characteristics. These findings suggest that Lactic Acid Bacteria (LAB) strains from different environments such as bee products can be the source for distinct EPS producer strains.
Açıklama
Anahtar Kelimeler
Exopolysaccharides, Bee isolates, LAB, Lactobacillus kunkeei, Dextran
Kaynak
Process Biochemistry
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
129
