Characterisation of dextran AP-27 produced by bee pollen isolate Lactobacillus kunkeei AP-27

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dc.contributor.authorYilmaz, Mustafa T.
dc.contributor.authorIspirli, Huemeyra
dc.contributor.authorAlidrisi, Hassan
dc.contributor.authorTaylan, Osman
dc.contributor.authorDertli, Enes
dc.date.accessioned2024-10-04T18:48:30Z
dc.date.available2024-10-04T18:48:30Z
dc.date.issued2023
dc.departmentBayburt Üniversitesien_US
dc.description.abstractAn exopolysaccharide (EPS) produced by bee pollen isolate Lactobacillus kunkeei AP-27 was characterised in this study. EPS monomeric composition of strain AP-27 was characterised by High-performance liquid chromatography (HPLC) analysis and glucose was the only sugar in the EPS repeating unit. Structural analysis of glucan AP27 by H-1 and C-13 Nuclear magnetic resonance (NMR) analysis demonstrated that this glucan was a dextran ((1-*6)-linked alpha-D-glucose units as the main body) consisting very low level of (1-* 4)-linked alpha-D-glucose units (similar to 0.5 %). Dextran AP-27 had a degradation temperature of 308 degrees C that revealed its superior thermal strength identified by Thermogravimetric (TGA) and Differential scanning calorimetry (DSC) analysis. The molecular weight of dextran AP-27 was determined to be 25 kDa by Gel Permeation Chromatography (GPC) analysis. Crystallographic properties of dextran AP-27 was determined by X-ray diffraction (XRD) analysis that divulged its amorphous feature. Scanning Electron Microscopy (SEM) analysis was applied for microstructural characterisation of dextran AP-27 which revealed its regular sphere like characteristics. These findings suggest that Lactic Acid Bacteria (LAB) strains from different environments such as bee products can be the source for distinct EPS producer strains.en_US
dc.description.sponsorshipDeanship of Scientific Research (DSR) at King Abdulaziz Uni- versity, Jeddah, Saudi Arabia [RG-22-135-42]en_US
dc.description.sponsorshipThe Deanship of Scientific Research (DSR) at King Abdulaziz Uni- versity, Jeddah, Saudi Arabia has funded this project, under grant no. RG-22-135-42.en_US
dc.identifier.doi10.1016/j.procbio.2023.03.007
dc.identifier.endpage29en_US
dc.identifier.issn1359-5113
dc.identifier.issn1873-3298
dc.identifier.scopus2-s2.0-85150812459en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage22en_US
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2023.03.007
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3074
dc.identifier.volume129en_US
dc.identifier.wosWOS:000978447300001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofProcess Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectExopolysaccharidesen_US
dc.subjectBee isolatesen_US
dc.subjectLABen_US
dc.subjectLactobacillus kunkeeien_US
dc.subjectDextranen_US
dc.titleCharacterisation of dextran AP-27 produced by bee pollen isolate Lactobacillus kunkeei AP-27en_US
dc.typeArticleen_US

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