Characterization of a 4,6???glucanotransferase from Lactobacillus reuteri E81 and production of malto-oligosaccharides with immune-modulatory roles
Küçük Resim Yok
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier B.V.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A wide number of Lactic Acid Bacteria (LAB) species produce ?-glucans with their ability to synthesize glucansucrases (GS) which use sucrose as substrate for the glucan production. Recently another group of enzymes in LAB gained special interest for their ability to produce ?-glucans targeting the substrates containing ?1-4-linkages and synthesizing new (?1-6) or (?1-3)–linkages as ??glucanotransferases. In this study, a putative 4,6???glucanotransferase (GTFB) from sourdough isolate Lactobacillus reuteri E81 was identified and expressed in Escherichia coli. The biochemical characterization of the GTFB-E81 confirmed its function as it cleaved the ?1-4-linkages in different substrates and produced new gluco-oligomers/polymers containing ?1-6 linkages together with the ?1-4-linkages detected by NMR analysis. GTFB-E81 produced malto-oligosaccharides targeting maltose and maltoheptaose as substrates with up to DP 8 detected by TLC and ESI-MS/MS analysis. The functional roles of these malto-oligosaccharides were determined by testing their immune-modulatory functions in HT29 cells and they triggered the production of anti-inflammatory 1L-4 and pro-inflammatory IL-12 cytokines. © 2018 Elsevier B.V.
Açıklama
Anahtar Kelimeler
4,6???Glucanotransferase, Immune-modulatory functions, Malto-oligosaccharides, 4,6 alpha glucanotransferase, glycosyltransferase, interleukin 12, interleukin 4, maltooligosaccharide, maltose, oligomer, oligosaccharide, polymer, unclassified drug, Article, bacterium identification, bacterium isolate, biochemical analysis, carbohydrate synthesis, controlled study, electrospray mass spectrometry, enzyme substrate, Escherichia coli, HT-29 cell line, human, human cell, immunomodulation, Lactobacillus reuteri, Lactobacillus reuteri E81, nonhuman, nuclear magnetic resonance, nucleotide sequence, protein cleavage, protein cross linking, protein function, thin layer chromatography, 4,6???Glucanotransferase, Immune-modulatory functions, Malto-oligosaccharides, 4,6 alpha glucanotransferase, glycosyltransferase, interleukin 12, interleukin 4, maltooligosaccharide, maltose, oligomer, oligosaccharide, polymer, unclassified drug, Article, bacterium identification, bacterium isolate, biochemical analysis, carbohydrate synthesis, controlled study, electrospray mass spectrometry, enzyme substrate, Escherichia coli, HT-29 cell line, human, human cell, immunomodulation, Lactobacillus reuteri, Lactobacillus reuteri E81, nonhuman, nuclear magnetic resonance, nucleotide sequence, protein cleavage, protein cross linking, protein function, thin layer chromatography
Kaynak
International Journal of Biological Macromolecules
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
124
