Characterization of a 4,6???glucanotransferase from Lactobacillus reuteri E81 and production of malto-oligosaccharides with immune-modulatory roles

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dc.authorid56781556400
dc.authorid8843724300
dc.authorid6701809848
dc.authorid6701802186
dc.authorid36815706500
dc.contributor.authorİspirli H.
dc.contributor.authorŞimşek Ö.
dc.contributor.authorSkory C.
dc.contributor.authorSağdıç O.
dc.contributor.authorDertli E.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:42:58Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:42:58Z
dc.date.issued2019
dc.departmentBayburt Üniversitesien_US
dc.description.abstractA wide number of Lactic Acid Bacteria (LAB) species produce ?-glucans with their ability to synthesize glucansucrases (GS) which use sucrose as substrate for the glucan production. Recently another group of enzymes in LAB gained special interest for their ability to produce ?-glucans targeting the substrates containing ?1-4-linkages and synthesizing new (?1-6) or (?1-3)–linkages as ??glucanotransferases. In this study, a putative 4,6???glucanotransferase (GTFB) from sourdough isolate Lactobacillus reuteri E81 was identified and expressed in Escherichia coli. The biochemical characterization of the GTFB-E81 confirmed its function as it cleaved the ?1-4-linkages in different substrates and produced new gluco-oligomers/polymers containing ?1-6 linkages together with the ?1-4-linkages detected by NMR analysis. GTFB-E81 produced malto-oligosaccharides targeting maltose and maltoheptaose as substrates with up to DP 8 detected by TLC and ESI-MS/MS analysis. The functional roles of these malto-oligosaccharides were determined by testing their immune-modulatory functions in HT29 cells and they triggered the production of anti-inflammatory 1L-4 and pro-inflammatory IL-12 cytokines. © 2018 Elsevier B.V.en_US
dc.identifier.doi10.1016/j.ijbiomac.2018.12.050
dc.identifier.endpage1219
dc.identifier.issn0141-8130
dc.identifier.pmid30529203en_US
dc.identifier.scopus2-s2.0-85057749968en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1213
dc.identifier.urihttps://dx.doi.org/10.1016/j.ijbiomac.2018.12.050
dc.identifier.urihttps://hdl.handle.net/20.500.12403/310
dc.identifier.volume124
dc.identifier.wosWOS:000456899300127en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject4,6???Glucanotransferase
dc.subjectImmune-modulatory functions
dc.subjectMalto-oligosaccharides
dc.subject4,6 alpha glucanotransferase
dc.subjectglycosyltransferase
dc.subjectinterleukin 12
dc.subjectinterleukin 4
dc.subjectmaltooligosaccharide
dc.subjectmaltose
dc.subjectoligomer
dc.subjectoligosaccharide
dc.subjectpolymer
dc.subjectunclassified drug
dc.subjectArticle
dc.subjectbacterium identification
dc.subjectbacterium isolate
dc.subjectbiochemical analysis
dc.subjectcarbohydrate synthesis
dc.subjectcontrolled study
dc.subjectelectrospray mass spectrometry
dc.subjectenzyme substrate
dc.subjectEscherichia coli
dc.subjectHT-29 cell line
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunomodulation
dc.subjectLactobacillus reuteri
dc.subjectLactobacillus reuteri E81
dc.subjectnonhuman
dc.subjectnuclear magnetic resonance
dc.subjectnucleotide sequence
dc.subjectprotein cleavage
dc.subjectprotein cross linking
dc.subjectprotein function
dc.subjectthin layer chromatography
dc.subject4,6???Glucanotransferase
dc.subjectImmune-modulatory functions
dc.subjectMalto-oligosaccharides
dc.subject4,6 alpha glucanotransferase
dc.subjectglycosyltransferase
dc.subjectinterleukin 12
dc.subjectinterleukin 4
dc.subjectmaltooligosaccharide
dc.subjectmaltose
dc.subjectoligomer
dc.subjectoligosaccharide
dc.subjectpolymer
dc.subjectunclassified drug
dc.subjectArticle
dc.subjectbacterium identification
dc.subjectbacterium isolate
dc.subjectbiochemical analysis
dc.subjectcarbohydrate synthesis
dc.subjectcontrolled study
dc.subjectelectrospray mass spectrometry
dc.subjectenzyme substrate
dc.subjectEscherichia coli
dc.subjectHT-29 cell line
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunomodulation
dc.subjectLactobacillus reuteri
dc.subjectLactobacillus reuteri E81
dc.subjectnonhuman
dc.subjectnuclear magnetic resonance
dc.subjectnucleotide sequence
dc.subjectprotein cleavage
dc.subjectprotein cross linking
dc.subjectprotein function
dc.subjectthin layer chromatography
dc.titleCharacterization of a 4,6???glucanotransferase from Lactobacillus reuteri E81 and production of malto-oligosaccharides with immune-modulatory rolesen_US
dc.typeArticleen_US

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