Comparison of telomere length and insulin-like growth factor-binding protein 7 promoter methylation between breast cancer tissues and adjacent normal tissues in Turkish women
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
John Wiley and Sons Inc.
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: Both insulin-like growth factor-binding protein 7 (IGFBP7) and telomere length (TL) are associated with proliferation and senescence of human breast cancer. This study assessed the clinical significance of both TL and IGFBP7 methylation status in breast cancer tissues compared with adjacent normal tissues. We also investigated whether IGFBP7 methylation status could be affecting TL. Methods: Telomere length was measured by quantitative PCR to compare tumors with their adjacent normal tissues. The IGFBP7 promoter methylation status was evaluated by methylation-specific PCR and its expression levels were determined by western blotting. Results: Telomeres were shorter in tumor tissues compared to controls (P<.0001). The mean TL was higher in breast cancer with invasive ductal carcinoma (IDC; n=72; P=.014) compared with other histological type (n=29), and TL in IDC with HER2 negative (n=53; P=.017) was higher than TL in IDC with HER2 positive (n=19). However, telomeres were shortened in advanced stages and growing tumors. IGFBP7 methylation was observed in 90% of tumor tissues and 59% of controls (P=.0002). Its frequency was significantly higher in IDC compared with invasive mixed carcinoma (IMC; P=.002) and it was not correlated either with protein expression or the other clinicopathological parameters. Conclusion: These results suggest that IGFBP7 promoter methylation and shorter TL in tumor compared with adjacent tissues may be predictive biomarkers for breast cancer. Telomere maintenance may be indicative of IDC and IDC with HER2 (?) of breast cancer. Further studies with larger number of cases are necessary to verify this association. © 2016 Wiley Periodicals, Inc.
Açıklama
Anahtar Kelimeler
breast cancer, insulin-like growth factor-binding protein 7, methylation, telomere, telomere length, insulin like growth factor binding protein 7, somatomedin binding protein, unclassified drug, insulin-like growth factor binding protein-related protein 1, somatomedin binding protein, adult, advanced cancer, Article, breast carcinoma, cancer grading, cancer growth, cancer staging, cancer tissue, controlled study, estrogen receptor positive breast cancer, family history, female, histology, human, human cell, human epidermal growth factor receptor 2 positive breast cancer, human tissue, major clinical study, menopause, mixed tumor, polymerase chain reaction, progesterone receptor positive breast cancer, protein expression, protein methylation, telomere length, telomere shortening, tumor invasion, Turk (people), Western blotting, breast, breast tumor, chemistry, cohort analysis, DNA methylation, genetics, middle aged, mortality, survival analysis, telomere, Breast, Breast Neoplasms, Cohort Studies, DNA Methylation, Female, Humans, Insulin-Like Growth Factor Binding Proteins, Middle Aged, Survival Analysis, Telomere, Turkey, breast cancer, insulin-like growth factor-binding protein 7, methylation, telomere, telomere length, insulin like growth factor binding protein 7, somatomedin binding protein, unclassified drug, insulin-like growth factor binding protein-related protein 1, somatomedin binding protein, adult, advanced cancer, Article, breast carcinoma, cancer grading, cancer growth, cancer staging, cancer tissue, controlled study, estrogen receptor positive breast cancer, family history, female, histology, human, human cell, human epidermal growth factor receptor 2 positive breast cancer, human tissue, major clinical study, menopause, mixed tumor, polymerase chain reaction, progesterone receptor positive breast cancer, protein expression, protein methylation, telomere length, telomere shortening, tumor invasion, Turk (people), Western blotting, breast, breast tumor, chemistry, cohort analysis, DNA methylation, genetics, middle aged, mortality, survival analysis, telomere, Breast, Breast Neoplasms, Cohort Studies, DNA Methylation, Female, Humans, Insulin-Like Growth Factor Binding Proteins, Middle Aged, Survival Analysis, Telomere, Turkey
Kaynak
Journal of Clinical Laboratory Analysis
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
31
Sayı
5
