Inhibition behaviours of some phenolic acids on rat kidney aldose reductase enzyme: an in vitro study
Küçük Resim Yok
Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor and Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Aldose reductase (AR) inhibitors have vital importance in the treatment and prevention of diabetic complications. In this study, rat kidney AR was purified 19.34-fold with a yield of 3.49% and a specific activity of 0.88 U/mg using DE-52 Cellulose anion exchange chromatography, gel filtration chromatography and 2?5? ADP Sepharose-4B affinity chromatography, respectively. After purification, the in vitro inhibition effects of some phenolic acids (tannic acid, chlorogenic acid, sinapic acid, protocatechuic acid, 4-hydroxybenzoic acid, p-coumaric acid, ferulic acid, vanillic acid, syringic acid, ?-resorcylic acid, 3-hydroxybenzoic acid and gallic acid) were investigated on purified enzyme. We determined IC50, Ki values and inhibition types of these phenolic acids. As a result, tannic and chlorogenic acid had a strong inhibition effect. On the other hand, gallic acid had a weak inhibition effect. In this study, all phenolic acids except for chlorogenic acid and p-coumaric acid showed non-competitive inhibition effects on rat kidney AR. © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Açıklama
Anahtar Kelimeler
Aldose reductase, diabetic complications, inhibition, phenolic acids, 3 hydroxybenzoic acid, 4 hydroxybenzoic acid, adenosine diphosphate, aldehyde reductase, alpha resorcylic acid, cellulose, chlorogenic acid, de 52 cellulose, ferulic acid, gallic acid, para coumaric acid, phenol derivative, protocatechuic acid, sepharose, sinapic acid, syringic acid, tannin, unclassified drug, vanillic acid, aldehyde reductase, hydroxybenzoic acid derivative, phenolic acid, affinity chromatography, anion exchange chromatography, antioxidant activity, Article, drug inhibition, enzyme activity, enzyme purification, gel filtration chromatography, IC50, in vitro study, kidney, nonhuman, priority journal, protein folding, rat, animal, antagonists and inhibitors, enzymology, kidney, polyacrylamide gel electrophoresis, Aldehyde Reductase, Animals, Electrophoresis, Polyacrylamide Gel, Hydroxybenzoates, In Vitro Techniques, Kidney, Rats, Aldose reductase, diabetic complications, inhibition, phenolic acids, 3 hydroxybenzoic acid, 4 hydroxybenzoic acid, adenosine diphosphate, aldehyde reductase, alpha resorcylic acid, cellulose, chlorogenic acid, de 52 cellulose, ferulic acid, gallic acid, para coumaric acid, phenol derivative, protocatechuic acid, sepharose, sinapic acid, syringic acid, tannin, unclassified drug, vanillic acid, aldehyde reductase, hydroxybenzoic acid derivative, phenolic acid, affinity chromatography, anion exchange chromatography, antioxidant activity, Article, drug inhibition, enzyme activity, enzyme purification, gel filtration chromatography, IC50, in vitro study, kidney, nonhuman, priority journal, protein folding, rat, animal, antagonists and inhibitors, enzymology, kidney, polyacrylamide gel electrophoresis, Aldehyde Reductase, Animals, Electrophoresis, Polyacrylamide Gel, Hydroxybenzoates, In Vitro Techniques, Kidney, Rats
Kaynak
Journal of Enzyme Inhibition and Medicinal Chemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
32
Sayı
1
