Inhibition behaviours of some phenolic acids on rat kidney aldose reductase enzyme: an in vitro study

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dc.authorid23391844000
dc.authorid56406261600
dc.authorid56711195000
dc.authorid6603903192
dc.contributor.authorAlim Z.
dc.contributor.authorKilinç N.
dc.contributor.authorŞengül B.
dc.contributor.authorBeydemir Ş.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:43:28Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:43:28Z
dc.date.issued2017
dc.departmentBayburt Üniversitesien_US
dc.description.abstractAldose reductase (AR) inhibitors have vital importance in the treatment and prevention of diabetic complications. In this study, rat kidney AR was purified 19.34-fold with a yield of 3.49% and a specific activity of 0.88 U/mg using DE-52 Cellulose anion exchange chromatography, gel filtration chromatography and 2?5? ADP Sepharose-4B affinity chromatography, respectively. After purification, the in vitro inhibition effects of some phenolic acids (tannic acid, chlorogenic acid, sinapic acid, protocatechuic acid, 4-hydroxybenzoic acid, p-coumaric acid, ferulic acid, vanillic acid, syringic acid, ?-resorcylic acid, 3-hydroxybenzoic acid and gallic acid) were investigated on purified enzyme. We determined IC50, Ki values and inhibition types of these phenolic acids. As a result, tannic and chlorogenic acid had a strong inhibition effect. On the other hand, gallic acid had a weak inhibition effect. In this study, all phenolic acids except for chlorogenic acid and p-coumaric acid showed non-competitive inhibition effects on rat kidney AR. © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.en_US
dc.identifier.doi10.1080/14756366.2016.1250752
dc.identifier.endpage284
dc.identifier.issn1475-6366
dc.identifier.issue1
dc.identifier.pmid28111996en_US
dc.identifier.scopus2-s2.0-85013846384en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage277
dc.identifier.urihttps://dx.doi.org/10.1080/14756366.2016.1250752
dc.identifier.urihttps://hdl.handle.net/20.500.12403/567
dc.identifier.volume32
dc.identifier.wosWOS:000392591100021en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor and Francis Ltd
dc.relation.ispartofJournal of Enzyme Inhibition and Medicinal Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAldose reductase
dc.subjectdiabetic complications
dc.subjectinhibition
dc.subjectphenolic acids
dc.subject3 hydroxybenzoic acid
dc.subject4 hydroxybenzoic acid
dc.subjectadenosine diphosphate
dc.subjectaldehyde reductase
dc.subjectalpha resorcylic acid
dc.subjectcellulose
dc.subjectchlorogenic acid
dc.subjectde 52 cellulose
dc.subjectferulic acid
dc.subjectgallic acid
dc.subjectpara coumaric acid
dc.subjectphenol derivative
dc.subjectprotocatechuic acid
dc.subjectsepharose
dc.subjectsinapic acid
dc.subjectsyringic acid
dc.subjecttannin
dc.subjectunclassified drug
dc.subjectvanillic acid
dc.subjectaldehyde reductase
dc.subjecthydroxybenzoic acid derivative
dc.subjectphenolic acid
dc.subjectaffinity chromatography
dc.subjectanion exchange chromatography
dc.subjectantioxidant activity
dc.subjectArticle
dc.subjectdrug inhibition
dc.subjectenzyme activity
dc.subjectenzyme purification
dc.subjectgel filtration chromatography
dc.subjectIC50
dc.subjectin vitro study
dc.subjectkidney
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectprotein folding
dc.subjectrat
dc.subjectanimal
dc.subjectantagonists and inhibitors
dc.subjectenzymology
dc.subjectkidney
dc.subjectpolyacrylamide gel electrophoresis
dc.subjectAldehyde Reductase
dc.subjectAnimals
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectHydroxybenzoates
dc.subjectIn Vitro Techniques
dc.subjectKidney
dc.subjectRats
dc.subjectAldose reductase
dc.subjectdiabetic complications
dc.subjectinhibition
dc.subjectphenolic acids
dc.subject3 hydroxybenzoic acid
dc.subject4 hydroxybenzoic acid
dc.subjectadenosine diphosphate
dc.subjectaldehyde reductase
dc.subjectalpha resorcylic acid
dc.subjectcellulose
dc.subjectchlorogenic acid
dc.subjectde 52 cellulose
dc.subjectferulic acid
dc.subjectgallic acid
dc.subjectpara coumaric acid
dc.subjectphenol derivative
dc.subjectprotocatechuic acid
dc.subjectsepharose
dc.subjectsinapic acid
dc.subjectsyringic acid
dc.subjecttannin
dc.subjectunclassified drug
dc.subjectvanillic acid
dc.subjectaldehyde reductase
dc.subjecthydroxybenzoic acid derivative
dc.subjectphenolic acid
dc.subjectaffinity chromatography
dc.subjectanion exchange chromatography
dc.subjectantioxidant activity
dc.subjectArticle
dc.subjectdrug inhibition
dc.subjectenzyme activity
dc.subjectenzyme purification
dc.subjectgel filtration chromatography
dc.subjectIC50
dc.subjectin vitro study
dc.subjectkidney
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectprotein folding
dc.subjectrat
dc.subjectanimal
dc.subjectantagonists and inhibitors
dc.subjectenzymology
dc.subjectkidney
dc.subjectpolyacrylamide gel electrophoresis
dc.subjectAldehyde Reductase
dc.subjectAnimals
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectHydroxybenzoates
dc.subjectIn Vitro Techniques
dc.subjectKidney
dc.subjectRats
dc.titleInhibition behaviours of some phenolic acids on rat kidney aldose reductase enzyme: an in vitro studyen_US
dc.typeArticleen_US

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