Impact of the exopolysaccharide layer on biofilms, adhesion and resistance to stress in Lactobacillus johnsonii FI9785
Küçük Resim Yok
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
BioMed Central Ltd.
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: The bacterial cell surface is a crucial factor in cell-cell and cell-host interactions. Lactobacillus johnsonii FI9785 produces an exopolysaccharide (EPS) layer whose quantity and composition is altered in mutants that harbour genetic changes in their eps gene clusters. We have assessed the effect of changes in EPS production on cell surface characteristics that may affect the ability of L. johnsonii to colonise the poultry host and exclude pathogens. Results: Analysis of physicochemical cell surface characteristics reflected by Zeta potential and adhesion to hexadecane showed that an increase in EPS gave a less negative, more hydrophilic surface and reduced autoaggregation. Autoaggregation was significantly higher in mutants that have reduced EPS, indicating that EPS can mask surface structures responsible for cell-cell interactions. EPS also affected biofilm formation, but here the quantity of EPS produced was not the only determinant. A reduction in EPS production increased bacterial adhesion to chicken gut explants, but made the bacteria less able to survive some stresses. Conclusions: This study showed that manipulation of EPS production in L. johnsonii FI9785 can affect properties which may improve its performance as a competitive exclusion agent, but that positive changes in adhesion may be compromised by a reduction in the ability to survive stress. © 2015 Dertli et al.
Açıklama
Anahtar Kelimeler
Adhesion, Biofilm, Cell surface, Exopolysaccharides, Lactobacillus johnsonii, antibiotic agent, exopolysaccharide, hexadecane, bacterial polysaccharide, animal cell, antibiotic resistance, Article, bacterial cell, bacterial strain, bacterial survival, bacterium adherence, bacterium culture, bacterium mutant, biofilm, cell aggregation, cell interaction, cell surface, chicken, controlled study, hydrophilicity, in vitro study, intestine, Lactobacillus johnsonii, nonhuman, physical chemistry, reduction, stress, zeta potential, animal, biofilm, growth, development and aging, Lactobacillus, metabolism, microbial viability, microbiology, physiological stress, physiology, Bacteria (microorganisms), Lactobacillus johnsonii, Animals, Bacterial Adhesion, Biofilms, Chickens, Intestines, Lactobacillus, Microbial Viability, Polysaccharides, Bacterial, Stress, Physiological, Adhesion, Biofilm, Cell surface, Exopolysaccharides, Lactobacillus johnsonii, antibiotic agent, exopolysaccharide, hexadecane, bacterial polysaccharide, animal cell, antibiotic resistance, Article, bacterial cell, bacterial strain, bacterial survival, bacterium adherence, bacterium culture, bacterium mutant, biofilm, cell aggregation, cell interaction, cell surface, chicken, controlled study, hydrophilicity, in vitro study, intestine, Lactobacillus johnsonii, nonhuman, physical chemistry, reduction, stress, zeta potential, animal, biofilm, growth, development and aging, Lactobacillus, metabolism, microbial viability, microbiology, physiological stress, physiology, Bacteria (microorganisms), Lactobacillus johnsonii, Animals, Bacterial Adhesion, Biofilms, Chickens, Intestines, Lactobacillus, Microbial Viability, Polysaccharides, Bacterial, Stress, Physiological
Kaynak
BMC Microbiology
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
15
Sayı
1
