Impact of the exopolysaccharide layer on biofilms, adhesion and resistance to stress in Lactobacillus johnsonii FI9785

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dc.authorid36815706500
dc.authorid7402464998
dc.authorid6603867601
dc.contributor.authorDertli E.
dc.contributor.authorMayer M.J.
dc.contributor.authorNarbad A.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:44:02Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:44:02Z
dc.date.issued2015
dc.departmentBayburt Üniversitesien_US
dc.description.abstractBackground: The bacterial cell surface is a crucial factor in cell-cell and cell-host interactions. Lactobacillus johnsonii FI9785 produces an exopolysaccharide (EPS) layer whose quantity and composition is altered in mutants that harbour genetic changes in their eps gene clusters. We have assessed the effect of changes in EPS production on cell surface characteristics that may affect the ability of L. johnsonii to colonise the poultry host and exclude pathogens. Results: Analysis of physicochemical cell surface characteristics reflected by Zeta potential and adhesion to hexadecane showed that an increase in EPS gave a less negative, more hydrophilic surface and reduced autoaggregation. Autoaggregation was significantly higher in mutants that have reduced EPS, indicating that EPS can mask surface structures responsible for cell-cell interactions. EPS also affected biofilm formation, but here the quantity of EPS produced was not the only determinant. A reduction in EPS production increased bacterial adhesion to chicken gut explants, but made the bacteria less able to survive some stresses. Conclusions: This study showed that manipulation of EPS production in L. johnsonii FI9785 can affect properties which may improve its performance as a competitive exclusion agent, but that positive changes in adhesion may be compromised by a reduction in the ability to survive stress. © 2015 Dertli et al.en_US
dc.identifier.doi10.1186/s12866-015-0347-2
dc.identifier.issn1471-2180
dc.identifier.issue1
dc.identifier.pmid25648083en_US
dc.identifier.scopus2-s2.0-84924182597en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://dx.doi.org/10.1186/s12866-015-0347-2
dc.identifier.urihttps://hdl.handle.net/20.500.12403/749
dc.identifier.volume15
dc.identifier.wosWOS:000349346500001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBioMed Central Ltd.
dc.relation.ispartofBMC Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAdhesion
dc.subjectBiofilm
dc.subjectCell surface
dc.subjectExopolysaccharides
dc.subjectLactobacillus johnsonii
dc.subjectantibiotic agent
dc.subjectexopolysaccharide
dc.subjecthexadecane
dc.subjectbacterial polysaccharide
dc.subjectanimal cell
dc.subjectantibiotic resistance
dc.subjectArticle
dc.subjectbacterial cell
dc.subjectbacterial strain
dc.subjectbacterial survival
dc.subjectbacterium adherence
dc.subjectbacterium culture
dc.subjectbacterium mutant
dc.subjectbiofilm
dc.subjectcell aggregation
dc.subjectcell interaction
dc.subjectcell surface
dc.subjectchicken
dc.subjectcontrolled study
dc.subjecthydrophilicity
dc.subjectin vitro study
dc.subjectintestine
dc.subjectLactobacillus johnsonii
dc.subjectnonhuman
dc.subjectphysical chemistry
dc.subjectreduction
dc.subjectstress
dc.subjectzeta potential
dc.subjectanimal
dc.subjectbiofilm
dc.subjectgrowth, development and aging
dc.subjectLactobacillus
dc.subjectmetabolism
dc.subjectmicrobial viability
dc.subjectmicrobiology
dc.subjectphysiological stress
dc.subjectphysiology
dc.subjectBacteria (microorganisms)
dc.subjectLactobacillus johnsonii
dc.subjectAnimals
dc.subjectBacterial Adhesion
dc.subjectBiofilms
dc.subjectChickens
dc.subjectIntestines
dc.subjectLactobacillus
dc.subjectMicrobial Viability
dc.subjectPolysaccharides, Bacterial
dc.subjectStress, Physiological
dc.subjectAdhesion
dc.subjectBiofilm
dc.subjectCell surface
dc.subjectExopolysaccharides
dc.subjectLactobacillus johnsonii
dc.subjectantibiotic agent
dc.subjectexopolysaccharide
dc.subjecthexadecane
dc.subjectbacterial polysaccharide
dc.subjectanimal cell
dc.subjectantibiotic resistance
dc.subjectArticle
dc.subjectbacterial cell
dc.subjectbacterial strain
dc.subjectbacterial survival
dc.subjectbacterium adherence
dc.subjectbacterium culture
dc.subjectbacterium mutant
dc.subjectbiofilm
dc.subjectcell aggregation
dc.subjectcell interaction
dc.subjectcell surface
dc.subjectchicken
dc.subjectcontrolled study
dc.subjecthydrophilicity
dc.subjectin vitro study
dc.subjectintestine
dc.subjectLactobacillus johnsonii
dc.subjectnonhuman
dc.subjectphysical chemistry
dc.subjectreduction
dc.subjectstress
dc.subjectzeta potential
dc.subjectanimal
dc.subjectbiofilm
dc.subjectgrowth, development and aging
dc.subjectLactobacillus
dc.subjectmetabolism
dc.subjectmicrobial viability
dc.subjectmicrobiology
dc.subjectphysiological stress
dc.subjectphysiology
dc.subjectBacteria (microorganisms)
dc.subjectLactobacillus johnsonii
dc.subjectAnimals
dc.subjectBacterial Adhesion
dc.subjectBiofilms
dc.subjectChickens
dc.subjectIntestines
dc.subjectLactobacillus
dc.subjectMicrobial Viability
dc.subjectPolysaccharides, Bacterial
dc.subjectStress, Physiological
dc.titleImpact of the exopolysaccharide layer on biofilms, adhesion and resistance to stress in Lactobacillus johnsonii FI9785en_US
dc.typeArticleen_US

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