Characterization of functional properties of enterococcus faecium strains isolated from human gut
Küçük Resim Yok
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
National Research Council of Canada
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this work was to characterize the functional properties of Enterococcus faecium strains identified after isolation from human faeces. Of these isolates, strain R13 showed the best resistance to low pH, bile salts, and survival in the simulated in vitro digestion assay, and demonstrated an important level of adhesion to hexadecane as a potential probiotic candidate. Analysis of the antibiotic resistance of E. faecium strains indicated that in general these isolates were sensitive to the tested antibiotics and no strain appeared to be resistant to vancomycin. Examination of the virulence determinants for E. faecium strains demonstrated that all strains contained the virulence genes common in gut-and food-originated enterococci, and strain R13 harboured the lowest number of virulence genes. Additionally, no strain contained the genes related to cytolysin metabolism and showed hemolytic activity. The antimicrobial role of E. faecium strains was tested against several pathogens, in which different levels of inhibitory effects were observed, and strain R13 was inhibitory to all tested pathogens. PCR screening of genes encoding enterocin A and B indicated the presence of these genes in E. faecium strains. Preliminary characterization of bacteriocins revealed that their activity was lost after proteolytic enzyme treatments, but no alteration in antimicrobial activity was observed at different pHs (3.5 to 9.5) and after heat treatments. In conclusion, this study revealed the functional characteristics of E. faecium R13 as a gut isolate, and this strain could be developed as a new probiotic after further tests. © 2015, National Research Council of Canada. All Rights Reserved.
Açıklama
Anahtar Kelimeler
Antibiotic resistance, Enterococcus faecium, Gut bacteria, Probiotic, Virulence determinants, bacterial protein, bacteriocin, bile salt, cytolysin, enterocin a, enterocin b, hexadecane, probiotic agent, proteinase, unclassified drug, vancomycin, antiinfective agent, bile acid, perforin, RNA 16S, vancomycin, virulence factor, anatomy, antibiotic resistance, antimicrobial activity, bacterium, blood, enzyme activity, feces, gene expression, hydrocarbon, metabolism, pathogen, pH, toxin, virulence, adhesion, adult, antibacterial activity, antibiotic resistance, antibiotic sensitivity, Article, bacterial gene, bacterial strain, bacterial virulence, bacterium identification, bacterium isolate, bacterium isolation, controlled study, Enterococcus faecium, feces, feces culture, genetic screening, human, human cell, in vitro study, intestine, nonhuman, normal human, pH, priority journal, antibiotic resistance, chemistry, drug effects, Enterococcus faecium, genetics, hemolysis, intestine, isolation and purification, metabolism, microbial sensitivity test, microbiology, pathogenicity, polymerase chain reaction, virulence, young adult, Enterococcus faecium, Adult, Anti-Infective Agents, Bacteriocins, Bile Acids and Salts, Drug Resistance, Microbial, Enterococcus faecium, Feces, Hemolysis, Humans, Hydrogen-Ion Concentration, Intestines, Microbial Sensitivity Tests, Perforin, Polymerase Chain Reaction, Probiotics, RNA, Ribosomal, 16S, Vancomycin, Virulence, Virulence Factors, Young Adult, Antibiotic resistance, Enterococcus faecium, Gut bacteria, Probiotic, Virulence determinants, bacterial protein, bacteriocin, bile salt, cytolysin, enterocin a, enterocin b, hexadecane, probiotic agent, proteinase, unclassified drug, vancomycin, antiinfective agent, bile acid, perforin, RNA 16S, vancomycin, virulence factor, anatomy, antibiotic resistance, antimicrobial activity, bacterium, blood, enzyme activity, feces, gene expression, hydrocarbon, metabolism, pathogen, pH, toxin, virulence, adhesion, adult, antibacterial activity, antibiotic resistance, antibiotic sensitivity, Article, bacterial gene, bacterial strain, bacterial virulence, bacterium identification, bacterium isolate, bacterium isolation, controlled study, Enterococcus faecium, feces, feces culture, genetic screening, human, human cell, in vitro study, intestine, nonhuman, normal human, pH, priority journal, antibiotic resistance, chemistry, drug effects, Enterococcus faecium, genetics, hemolysis, intestine, isolation and purification, metabolism, microbial sensitivity test, microbiology, pathogenicity, polymerase chain reaction, virulence, young adult, Enterococcus faecium, Adult, Anti-Infective Agents, Bacteriocins, Bile Acids and Salts, Drug Resistance, Microbial, Enterococcus faecium, Feces, Hemolysis, Humans, Hydrogen-Ion Concentration, Intestines, Microbial Sensitivity Tests, Perforin, Polymerase Chain Reaction, Probiotics, RNA, Ribosomal, 16S, Vancomycin, Virulence, Virulence Factors, Young Adult
Kaynak
Canadian Journal of Microbiology
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
61
Sayı
11
