Characterization of functional properties of enterococcus faecium strains isolated from human gut

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dc.authorid56781556400
dc.authorid56926613800
dc.authorid36815706500
dc.contributor.authorİspirli H.
dc.contributor.authorDemirbaş F.
dc.contributor.authorDertli E.
dc.date.accessioned20.04.201910:49:12
dc.date.accessioned2019-04-20T21:43:55Z
dc.date.available20.04.201910:49:12
dc.date.available2019-04-20T21:43:55Z
dc.date.issued2015
dc.departmentBayburt Üniversitesien_US
dc.description.abstractThe aim of this work was to characterize the functional properties of Enterococcus faecium strains identified after isolation from human faeces. Of these isolates, strain R13 showed the best resistance to low pH, bile salts, and survival in the simulated in vitro digestion assay, and demonstrated an important level of adhesion to hexadecane as a potential probiotic candidate. Analysis of the antibiotic resistance of E. faecium strains indicated that in general these isolates were sensitive to the tested antibiotics and no strain appeared to be resistant to vancomycin. Examination of the virulence determinants for E. faecium strains demonstrated that all strains contained the virulence genes common in gut-and food-originated enterococci, and strain R13 harboured the lowest number of virulence genes. Additionally, no strain contained the genes related to cytolysin metabolism and showed hemolytic activity. The antimicrobial role of E. faecium strains was tested against several pathogens, in which different levels of inhibitory effects were observed, and strain R13 was inhibitory to all tested pathogens. PCR screening of genes encoding enterocin A and B indicated the presence of these genes in E. faecium strains. Preliminary characterization of bacteriocins revealed that their activity was lost after proteolytic enzyme treatments, but no alteration in antimicrobial activity was observed at different pHs (3.5 to 9.5) and after heat treatments. In conclusion, this study revealed the functional characteristics of E. faecium R13 as a gut isolate, and this strain could be developed as a new probiotic after further tests. © 2015, National Research Council of Canada. All Rights Reserved.en_US
dc.identifier.doi10.1139/cjm-2015-0446
dc.identifier.endpage870
dc.identifier.issn0008-4166
dc.identifier.issue11
dc.identifier.pmid26485327en_US
dc.identifier.scopus2-s2.0-84945393877en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage861
dc.identifier.urihttps://dx.doi.org/10.1139/cjm-2015-0446
dc.identifier.urihttps://hdl.handle.net/20.500.12403/718
dc.identifier.volume61
dc.identifier.wosWOS:000363906200008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherNational Research Council of Canada
dc.relation.ispartofCanadian Journal of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntibiotic resistance
dc.subjectEnterococcus faecium
dc.subjectGut bacteria
dc.subjectProbiotic
dc.subjectVirulence determinants
dc.subjectbacterial protein
dc.subjectbacteriocin
dc.subjectbile salt
dc.subjectcytolysin
dc.subjectenterocin a
dc.subjectenterocin b
dc.subjecthexadecane
dc.subjectprobiotic agent
dc.subjectproteinase
dc.subjectunclassified drug
dc.subjectvancomycin
dc.subjectantiinfective agent
dc.subjectbile acid
dc.subjectperforin
dc.subjectRNA 16S
dc.subjectvancomycin
dc.subjectvirulence factor
dc.subjectanatomy
dc.subjectantibiotic resistance
dc.subjectantimicrobial activity
dc.subjectbacterium
dc.subjectblood
dc.subjectenzyme activity
dc.subjectfeces
dc.subjectgene expression
dc.subjecthydrocarbon
dc.subjectmetabolism
dc.subjectpathogen
dc.subjectpH
dc.subjecttoxin
dc.subjectvirulence
dc.subjectadhesion
dc.subjectadult
dc.subjectantibacterial activity
dc.subjectantibiotic resistance
dc.subjectantibiotic sensitivity
dc.subjectArticle
dc.subjectbacterial gene
dc.subjectbacterial strain
dc.subjectbacterial virulence
dc.subjectbacterium identification
dc.subjectbacterium isolate
dc.subjectbacterium isolation
dc.subjectcontrolled study
dc.subjectEnterococcus faecium
dc.subjectfeces
dc.subjectfeces culture
dc.subjectgenetic screening
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectintestine
dc.subjectnonhuman
dc.subjectnormal human
dc.subjectpH
dc.subjectpriority journal
dc.subjectantibiotic resistance
dc.subjectchemistry
dc.subjectdrug effects
dc.subjectEnterococcus faecium
dc.subjectgenetics
dc.subjecthemolysis
dc.subjectintestine
dc.subjectisolation and purification
dc.subjectmetabolism
dc.subjectmicrobial sensitivity test
dc.subjectmicrobiology
dc.subjectpathogenicity
dc.subjectpolymerase chain reaction
dc.subjectvirulence
dc.subjectyoung adult
dc.subjectEnterococcus faecium
dc.subjectAdult
dc.subjectAnti-Infective Agents
dc.subjectBacteriocins
dc.subjectBile Acids and Salts
dc.subjectDrug Resistance, Microbial
dc.subjectEnterococcus faecium
dc.subjectFeces
dc.subjectHemolysis
dc.subjectHumans
dc.subjectHydrogen-Ion Concentration
dc.subjectIntestines
dc.subjectMicrobial Sensitivity Tests
dc.subjectPerforin
dc.subjectPolymerase Chain Reaction
dc.subjectProbiotics
dc.subjectRNA, Ribosomal, 16S
dc.subjectVancomycin
dc.subjectVirulence
dc.subjectVirulence Factors
dc.subjectYoung Adult
dc.subjectAntibiotic resistance
dc.subjectEnterococcus faecium
dc.subjectGut bacteria
dc.subjectProbiotic
dc.subjectVirulence determinants
dc.subjectbacterial protein
dc.subjectbacteriocin
dc.subjectbile salt
dc.subjectcytolysin
dc.subjectenterocin a
dc.subjectenterocin b
dc.subjecthexadecane
dc.subjectprobiotic agent
dc.subjectproteinase
dc.subjectunclassified drug
dc.subjectvancomycin
dc.subjectantiinfective agent
dc.subjectbile acid
dc.subjectperforin
dc.subjectRNA 16S
dc.subjectvancomycin
dc.subjectvirulence factor
dc.subjectanatomy
dc.subjectantibiotic resistance
dc.subjectantimicrobial activity
dc.subjectbacterium
dc.subjectblood
dc.subjectenzyme activity
dc.subjectfeces
dc.subjectgene expression
dc.subjecthydrocarbon
dc.subjectmetabolism
dc.subjectpathogen
dc.subjectpH
dc.subjecttoxin
dc.subjectvirulence
dc.subjectadhesion
dc.subjectadult
dc.subjectantibacterial activity
dc.subjectantibiotic resistance
dc.subjectantibiotic sensitivity
dc.subjectArticle
dc.subjectbacterial gene
dc.subjectbacterial strain
dc.subjectbacterial virulence
dc.subjectbacterium identification
dc.subjectbacterium isolate
dc.subjectbacterium isolation
dc.subjectcontrolled study
dc.subjectEnterococcus faecium
dc.subjectfeces
dc.subjectfeces culture
dc.subjectgenetic screening
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectintestine
dc.subjectnonhuman
dc.subjectnormal human
dc.subjectpH
dc.subjectpriority journal
dc.subjectantibiotic resistance
dc.subjectchemistry
dc.subjectdrug effects
dc.subjectEnterococcus faecium
dc.subjectgenetics
dc.subjecthemolysis
dc.subjectintestine
dc.subjectisolation and purification
dc.subjectmetabolism
dc.subjectmicrobial sensitivity test
dc.subjectmicrobiology
dc.subjectpathogenicity
dc.subjectpolymerase chain reaction
dc.subjectvirulence
dc.subjectyoung adult
dc.subjectEnterococcus faecium
dc.subjectAdult
dc.subjectAnti-Infective Agents
dc.subjectBacteriocins
dc.subjectBile Acids and Salts
dc.subjectDrug Resistance, Microbial
dc.subjectEnterococcus faecium
dc.subjectFeces
dc.subjectHemolysis
dc.subjectHumans
dc.subjectHydrogen-Ion Concentration
dc.subjectIntestines
dc.subjectMicrobial Sensitivity Tests
dc.subjectPerforin
dc.subjectPolymerase Chain Reaction
dc.subjectProbiotics
dc.subjectRNA, Ribosomal, 16S
dc.subjectVancomycin
dc.subjectVirulence
dc.subjectVirulence Factors
dc.subjectYoung Adult
dc.titleCharacterization of functional properties of enterococcus faecium strains isolated from human guten_US
dc.typeArticleen_US

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