Investigation of the recovery efficiency of CeUF method through RT-qPCR quantification of inactivated SARS-CoV-2 in untreated wastewater
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Wastewater-based disease monitoring is an early warning system and a surveillance tool for infectious disease outbreaks regarding pathogens with pandemic potential. This study aimed at investigating the recovery efficiency of centrifugal ultrafiltration (CeUF), which is one of the most-used virus concentration methods, for inactive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) added to wastewater. Inactivated SARS-CoV-2 was inoculated into untreated wastewater at different concentrations (4 x 10(3), 8 x 10(3), 16 x 10(3), 24 x 10(3) and 32 x 10(3) gene copy/mu L) and concentrated through ultrafiltration with a disposable centrifugal filter device. Total nucleic acids in concentrated filtrates were extracted and isolated by an automated system. In isolates, total RNA was measured by a UV/VIS spectrophotometer, and the recovered virus was quantified by RT-qPCR with two gene regions (N1 and N2). The recovery rates were between 11% and 17.8% (mean 15.1%, CV below 15%). While there were positive correlations among the inoculated virus, total RNA and recovered virus, there was no correlation and linearity between the recovery rates. Despite limited recovery rates, CeUF integrated with RT-qPCR quantification can be a valid assay for monitoring SARS-CoV-2 in wastewater, and an early warning system.
Açıklama
Anahtar Kelimeler
Coronavirus, RT-qPCR, SARS-CoV-2, Ultrafiltration, Wastewater
Kaynak
Desalination and Water Treatment
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
262
