Investigation of the recovery efficiency of CeUF method through RT-qPCR quantification of inactivated SARS-CoV-2 in untreated wastewater

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dc.authoridSait, Ahmet/0000-0001-7658-8793
dc.contributor.authorSait, Ahmet
dc.contributor.authorKorkmaz, Serol
dc.contributor.authorParmaksiz, Ayse
dc.contributor.authorBayraktar, Bulent
dc.date.accessioned2024-10-04T18:51:16Z
dc.date.available2024-10-04T18:51:16Z
dc.date.issued2022
dc.departmentBayburt Üniversitesien_US
dc.description.abstractWastewater-based disease monitoring is an early warning system and a surveillance tool for infectious disease outbreaks regarding pathogens with pandemic potential. This study aimed at investigating the recovery efficiency of centrifugal ultrafiltration (CeUF), which is one of the most-used virus concentration methods, for inactive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) added to wastewater. Inactivated SARS-CoV-2 was inoculated into untreated wastewater at different concentrations (4 x 10(3), 8 x 10(3), 16 x 10(3), 24 x 10(3) and 32 x 10(3) gene copy/mu L) and concentrated through ultrafiltration with a disposable centrifugal filter device. Total nucleic acids in concentrated filtrates were extracted and isolated by an automated system. In isolates, total RNA was measured by a UV/VIS spectrophotometer, and the recovered virus was quantified by RT-qPCR with two gene regions (N1 and N2). The recovery rates were between 11% and 17.8% (mean 15.1%, CV below 15%). While there were positive correlations among the inoculated virus, total RNA and recovered virus, there was no correlation and linearity between the recovery rates. Despite limited recovery rates, CeUF integrated with RT-qPCR quantification can be a valid assay for monitoring SARS-CoV-2 in wastewater, and an early warning system.en_US
dc.identifier.doi10.5004/dwt.2022.28434
dc.identifier.endpage59en_US
dc.identifier.issn1944-3994
dc.identifier.issn1944-3986
dc.identifier.scopus2-s2.0-85135219147en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage54en_US
dc.identifier.urihttps://doi.org/10.5004/dwt.2022.28434
dc.identifier.urihttp://hdl.handle.net/20.500.12403/3446
dc.identifier.volume262en_US
dc.identifier.wosWOS:000882984900005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofDesalination and Water Treatmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCoronavirusen_US
dc.subjectRT-qPCRen_US
dc.subjectSARS-CoV-2en_US
dc.subjectUltrafiltrationen_US
dc.subjectWastewateren_US
dc.titleInvestigation of the recovery efficiency of CeUF method through RT-qPCR quantification of inactivated SARS-CoV-2 in untreated wastewateren_US
dc.typeArticleen_US

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